• Western blot analysis of extracts of various cell lines, using CDH12 antibody (STJ112244) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CDH12 antibody (390-540) (STJ112244)

SKU:
STJ112244

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CDH12 (390-540) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDH12
Gene ID: 1010
Uniprot ID: CAD12_HUMAN
Immunogen Region: 390-540
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 390-540 of human CDH12 (NP_004052.2).
Immunogen Sequence: TMEVYEDTPVGTIIGAVTAQ DLDVGSSAVRYFIDWKSDGD SYFTIDGNEGTIATNELLDR ESTAQYNFSIIASKVSNPLL TSKVNILINVLDVNEFPPEI SVPYETAVCENAKPGQIIQI VSAADRDLSPAGQQFSFRLS PEAAIKPNFTV
Tissue Specificity Brain.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types.
Protein Name Cadherin-12
Brain Cadherin
Br-Cadherin
Neural Type Cadherin 2
N-Cadherin 2
Database Links Reactome: R-HSA-418990
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cadherin-12 antibody
Anti-Brain Cadherin antibody
Anti-Br-Cadherin antibody
Anti-Neural Type Cadherin 2 antibody
Anti-N-Cadherin 2 antibody
Anti-CDH12 antibody

Information sourced from Uniprot.org

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