| Host: |
Mouse |
| Applications: |
WB/ICC/IHC/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-E-Cadherin (Cytoplasmic) is suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
M168 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: |
Protein A Purified |
| Dilution Range: |
WB 1:1000IHC 1:50ICC 1:250IP 1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Cdh1 |
| Gene ID: |
12550 |
| Uniprot ID: |
CADH1_MOUSE |
| Immunogen Region: |
Cytoplasmic |
| Specificity: |
This E-cadherin antibody detects a 120 kDa* protein in human A431 cells, and does not cross-react with VE-cadherin or N-cadherin. |
| Immunogen: |
Clone (M168) was generated from a mouse recombinant E-Cadherin protein containing amino acids in the cytoplasmic region. This sequence is highly conserved in human and rat E-cadherin. |
| Tissue Specificity | Expressed in inner and outer pillar cells of the organ of Corti (at protein level). Non-neural epithelial tissues. |
| Post Translational Modifications | During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation. O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Ser-287 and Thr-511 are O-manosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3. N-glycosylation at Asn-639 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location. Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-756. |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA).mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria. |
| Protein Name | Cadherin-1Arc-1Epithelial CadherinE-CadherinUvomorulinCd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 |
| Database Links | Reactome: R-MMU-1474228Reactome: -MMU-216083Reactome: -MMU-351906Reactome: -MMU-418990Reactome: -MMU-5626467 |
| Cellular Localisation | Cell JunctionAdherens JunctionCell MembraneSingle-Pass Type I Membrane ProteinEndosomeGolgi ApparatusTrans-Golgi NetworkColocalizes With Dlgap5 At Sites Of Cell-Cell Contact In Intestinal Epithelial CellsAnchored To Actin Microfilaments Through Association With Alpha-Beta- And Gamma-CateninSequential Proteolysis Induced By Apoptosis Or Calcium InfluxResults In Translocation From Sites Of Cell-Cell Contact To The CytoplasmColocalizes With Rab11a Endosomes During Its Transport From The Golgi Apparatus To The Plasma Membrane |
| Alternative Antibody Names | Anti-Cadherin-1 antibodyAnti-Arc-1 antibodyAnti-Epithelial Cadherin antibodyAnti-E-Cadherin antibodyAnti-Uvomorulin antibodyAnti-Cd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 antibodyAnti-Cdh1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
