| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-CD74 (159-171) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: This antibody was observed to recognize variations of bands at approx. 26kDa, 30kDa, 35kDa and 70kDa in Human Duodenum and Lung lysates (calculated MW of 26.4kDa according to NP_00434 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CD74 |
| Gene ID: | 972 |
| Uniprot ID: | HG2A_HUMAN |
| Immunogen Region: | 159-171 |
| Accession Number: | NP_001020330.1; NP_004346.1 |
| Specificity: | This antibody is expected to recognize isoform a (NP_001020330.1) and isoform b (NP_004346.1). |
| Immunogen Sequence: | KGSFPENLRHLKN |
| Post Translational Modifications | O-glycosylated with core 1 or possibly core 8 glycans. Contains chondroitin sulfate. |
| Function | Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. Class-II-associated invariant chain peptide: Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome. Isoform p41: Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2. Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform. |
| Protein Name | Hla Class Ii Histocompatibility Antigen Gamma Chain Hla-Dr Antigens-Associated Invariant Chain Ia Antigen-Associated Invariant Chain Ii Cd Antigen Cd74 Cleaved Into - Class-Ii-Associated Invariant Chain Peptide Clip |
| Database Links | Reactome: R-HSA-202733 Reactome: R-HSA-2132295 |
| Cellular Localisation | Cell Membrane Single-Pass Type Ii Membrane Protein Endoplasmic Reticulum Membrane Golgi Apparatus Trans-Golgi Network Endosome Lysosome Secreted Transits Through A Number Of Intracellular Compartments In The Endocytic Pathway It Can Either Undergo Proteolysis Or Reach The Cell Membrane Isoform P41: Late Endosome |
| Alternative Antibody Names | Anti-Hla Class Ii Histocompatibility Antigen Gamma Chain antibody Anti-Hla-Dr Antigens-Associated Invariant Chain antibody Anti-Ia Antigen-Associated Invariant Chain antibody Anti-Ii antibody Anti-Cd Antigen Cd74 Cleaved Into - Class-Ii-Associated Invariant Chain Peptide antibody Anti-Clip antibody Anti-CD74 antibody Anti-DHLAG antibody |
Information sourced from Uniprot.org