• Immunohistochemistry analysis of paraffin-embedded human lymph node tissue, showing membrane and cytoplasmic localization with DAB staining using CD45 monoclonal antibody.
  • Western blot analysis using CD45 monoclonal antibody against truncated CD45 recombinant protein (1).

Anti-CD45 antibody (STJ97930)

Current Stock:
Host: Mouse
Applications: WB, IHC-P, ELISA
Reactivity: Human
Short Description: Mouse monoclonal primary antibody against CD45.
Clonality: Monoclonal
Clone ID: 4A8A4C7A2
Conjugation: Unconjugated
Formulation: Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.
Purification: Affinity purification
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/mL
Isotype: IgG1
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
Specificity: CD45 monoclonal antibody detects endogenous levels of CD45 protein.
Gene ID: 5788
Gene Symbol: PTPRC
Immunogen: Purified recombinant fragment of CD45 expressed in E. Coli.
Tissue Specificity Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.
Post Translational Modifications Heavily N- and O-glycosylated.
Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
Database Links Reactome: R-HSA-202427
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Colocalized With Dpp4 In Membrane Rafts
Protein Name Receptor-Type Tyrosine-Protein Phosphatase C
Leukocyte Common Antigen
Cd Antigen Cd45
Alternative Names Anti-Receptor-Type Tyrosine-Protein Phosphatase C antibody
Anti-Leukocyte Common Antigen antibody
Anti-L-Ca antibody
Anti-T200 antibody
Anti-Cd Antigen Cd45 antibody
Anti-PTPRC antibody
Anti-CD45 antibody

Information sourced from Uniprot.org

6 months standard