• Western blot analysis of the lysates from HUVECcells using CD226/DNAM-1 antibody.
  • Western blot analysis of lysates from COS7 and K562 cells, treated with PMA 125ng/ml 30', using CD226/DNAM-1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of HeLa cells, using CD226/DNAM-1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of AD293 using DNAM-1 Polyclonal Antibody diluted at 1：500
  • Western blot analysis of mouse-brain cells using DNAM-1 Polyclonal Antibody diluted at 1：500
  • Western blot analysis of COS7 cells using DNAM-1 Polyclonal Antibody diluted at 1：500

Anti-CD226 antibody (287-336 aa) (STJ92746)

SKU:
STJ92746

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CD226 antigen (287-336 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CD226
Gene ID: 10666
Uniprot ID: CD226_HUMAN
Immunogen Region: 287-336 aa
Specificity: DNAM-1 Polyclonal Antibody detects endogenous levels of DNAM-1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CD226/DNAM-1 at the amino acid range 287-336
Post Translational Modifications PKC-mediated phosphorylation at Ser-329 is required for lipid raft recruitment. Phosphorylation of Tyr-322 requires association with lipid rafts and allows GRB2 binding and activation of subsequent signaling.
Function Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes.
Protein Name Cd226 Antigen
Dnax Accessory Molecule 1
Dnam-1
Cd Antigen Cd226
Database Links Reactome: R-HSA-198933
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Localizes To Lipid Rafts
Alternative Antibody Names Anti-Cd226 Antigen antibody
Anti-Dnax Accessory Molecule 1 antibody
Anti-Dnam-1 antibody
Anti-Cd Antigen Cd226 antibody
Anti-CD226 antibody
Anti-DNAM1 antibody

Information sourced from Uniprot.org

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