| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-CD226 antigen (287-336 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CD226 |
| Gene ID: | 10666 |
| Uniprot ID: | CD226_HUMAN |
| Immunogen Region: | 287-336 aa |
| Specificity: | DNAM-1 Polyclonal Antibody detects endogenous levels of DNAM-1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human CD226/DNAM-1 at the amino acid range 287-336 |
| Post Translational Modifications | PKC-mediated phosphorylation at Ser-329 is required for lipid raft recruitment. Phosphorylation of Tyr-322 requires association with lipid rafts and allows GRB2 binding and activation of subsequent signaling. |
| Function | Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes. |
| Protein Name | Cd226 Antigen Dnax Accessory Molecule 1 Dnam-1 Cd Antigen Cd226 |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Localizes To Lipid Rafts |
| Alternative Antibody Names | Anti-Cd226 Antigen antibody Anti-Dnax Accessory Molecule 1 antibody Anti-Dnam-1 antibody Anti-Cd Antigen Cd226 antibody Anti-CD226 antibody Anti-DNAM1 antibody |
Information sourced from Uniprot.org