||WB, IHC, IF
||Human, Mouse, Rat
||FOR RESEARCH USE ONLY (RUO).
||Rabbit polyclonal antibody anti-CAD is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
||WB 1:500-1:2000IHC 1:50-1:100IF 1:50-1:200
||Store at-20°C. Avoid freeze/thaw cycles.
||Recombinant fusion protein containing a sequence corresponding to amino acids 1650-1900 of human CAD (NP_004332.2).
| Post Translational Modifications || Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivity to feedback inhibition by UTP. |
| Function || This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase). |
| Protein Name || Cad Protein Includes - Glutamine-Dependent Carbamoyl-Phosphate Synthase - Aspartate Carbamoyltransferase - Dihydroorotase |
| Database Links || Reactome: R-HSA-500753 |
| Cellular Localisation || CytoplasmNucleusCytosolic And Unphosphorylated In Resting CellsTranslocates To The Nucleus In Response To Egf StimulationNuclear Import Promotes Optimal Cell Growth |
| Alternative Antibody Names || Anti-Cad Protein Includes - Glutamine-Dependent Carbamoyl-Phosphate Synthase - Aspartate Carbamoyltransferase - Dihydroorotase antibodyAnti-CAD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance