• Western blot analysis of extracts of HepG2 cells, using C5/C5a antibody (STJ110403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of lysates from Mouse lung, using C5/C5a Rabbit polyclonal antibody (STJ110403) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of Human plasma, using C5/C5a antibody (STJ110403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of U2OS cells using C5/C5a antibody (STJ110403) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-C5 antibody (1567-1676) (STJ110403)

SKU:
STJ110403

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-C5/C5a (1567-1676) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: C5
Gene ID: 727
Uniprot ID: CO5_HUMAN
Immunogen Region: 1567-1676
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1567-1676 of human C5/C5a (NP_001726.2).
Immunogen Sequence: SITVENVFVKYKATLLDIYK TGEAVAEKDSEITFIKKVTC TNAELVKGRQYLIMGKEALQ IKYNFSFRYIYPLDSLTWIE YWPRDTTCSSCQAFLANLDE FAEDIFLNGC
Function Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. C5a anaphylatoxin: Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Protein Name Complement C5
C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 4 Cleaved Into - Complement C5 Beta Chain - Complement C5 Alpha Chain - C5a Anaphylatoxin - Complement C5 Alpha' Chain
Database Links Reactome: R-HSA-166665
Reactome: R-HSA-174577
Reactome: R-HSA-375276
Reactome: R-HSA-418594
Reactome: R-HSA-977606
Cellular Localisation Secreted
Alternative Antibody Names Anti-Complement C5 antibody
Anti-C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 4 Cleaved Into - Complement C5 Beta Chain - Complement C5 Alpha Chain - C5a Anaphylatoxin - Complement C5 Alpha' Chain antibody
Anti-C5 antibody
Anti-CPAMD4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance