| Short Description : | Rabbit polyclonal BRD3 (150-305) antibody for use in WB, IHC-P and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BRD3 |
| Gene ID: | 8019 |
| Uniprot ID: | BRD3_HUMAN |
| Immunogen Region: | 150-305 |
| Immunogen Sequence: | PAPKGKGRKPAAGAQSAGTQ QVAAVSSVSPATPFQSVPPT VSQTPVIAATPVPTITANVT SVPVPPAAAPPPPATPIVPV VPPTPPVVKKKGVKRKADTT TPTTSAITASRSESPPPLSD PKQAKVVARRESGGRPIKPP KKDLEDGEVPQHAGKK |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 150-305 of human BRD3 (NP_031397.1). |
| Tissue Specificity | Ubiquitous. |
| Function | Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures. Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation. In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4. Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression. Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets. |
| Protein Name | Bromodomain-Containing Protein 3Ring3-Like Protein |
| Database Links | |
| Cellular Localisation | NucleusChromosomeDetected On Chromatin |
| Alternative Antibody Names | Anti-Bromodomain-Containing Protein 3 antibodyAnti-Ring3-Like Protein antibodyAnti-BRD3 antibodyAnti-KIAA0043 antibodyAnti-RING3L antibody |
Information sourced from Uniprot.org
