• Western blot detection of beta Tubulin in Chlamydomonas cell lysates using beta Tubulin mouse mAb (1:1000 diluted).Predicted band size:55KDa.Observed band size:55KDa.
  • Western blot detection of Tubulin beta in Goat skeletal muscle, CHO-k1, COS7, C6, 3T3 and K562 cell lysates using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size:55KDa.Observed band size:55KDa.Exposure time:10sec.
  • Western blot detection of Tubulin beta in goat intestine, goat skeletal muscle, goat spleen and goat lung cell lysates using Tubulin beta mouse mAb (1:5000-1:10000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.
  • Tubulin beta antibody’s stability test under 37℃ 50 hours compare to 4℃ 50 hours.
  • Immunocytochemistry staining of HeLa cells fixed with 4% paraformaldehyde and using anti-Tubulin beta mouse mAb (dilution 1:500).
  • Western blot detection of Tubulin beta in COS7, LNCap, MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.

Anti-Beta-Tubulin antibody (STJ99132)

SKU:
STJ99132
Current Stock:
Host: Mouse
Applications: WB, IF
Reactivity: Human,Mouse,Monkey,Goat,Hamster,Rat,Chlamydomonas
Short Description: Mouse monoclonal primary antibody against Beta-Tubulin.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 1E1-E8-H4
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG1
Dilution Range: WB 1:5000
ICC 1:500
Specificity: This antibody detects endogenous levels of Tubulin beta and does not cross-react with related proteins.
Uniprot ID: TBB5_HUMAN
Gene ID: 203068
Gene Symbol: TUBB
Immunogen: Purified recombinant human Tubulin beta protein fragments expressed in E.coli.
Tissue Specificity Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Post Translational Modifications Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Database Links Reactome: R-HSA-2565942
Cellular Localisation Cytoplasm
Cytoskeleton
Protein Name Tubulin Beta Chain
Tubulin Beta-5 Chain
Alternative Names Anti-Tubulin Beta Chain antibody
Anti-Tubulin Beta-5 Chain antibody
Anti-TUBB antibody
Anti-TUBB5 antibody
Anti-OK antibody
Anti-SW-cl.56 antibody

Information sourced from uniprot.org

6 months standard