• Western blot analysis of extracts of various cell lines, using B3GALNT1 antibody (STJ110066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-B3GALNT1 antibody (44-331) (STJ110066)

SKU:
STJ110066

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-B3GALNT1 (44-331) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: B3GALNT1
Gene ID: 8706
Uniprot ID: B3GL1_HUMAN
Immunogen Region: 44-331
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 44-331 of human B3GALNT1 (NP_003772.1).
Immunogen Sequence: IERVNWMYFYEYEPIYRQDF HFTLREHSNCSHQNPFLVIL VTSHPSDVKARQAIRVTWGE KKSWWGYEVLTFFLLGQEAE KEDKMLALSLEDEHLLYGDI IRQDFLDTYNNLTLKTIMAF RWVTEFCPNAKYVMKTDTDV FINTGNLVKYLLNLNHSEKF FTGYPLIDNYSYRGFYQKTH ISYQEYPFKVFPPYCSGLGY IMSRDLVPRIYEMMGHVKPI KFEDVYVGICLNLLKVNIH
Tissue Specificity Higher expression in heart and brain, and to a lesser extent in lung, placenta, kidney and testis. Lower expression in liver, spleen and stomach. No expression in skeletal muscle.
Function Transfers N-acetylgalactosamine onto globotriaosylceramide. Plays a critical role in preimplantation stage embryonic development.
Protein Name Udp-Galnac -Beta-1 -3-N-Acetylgalactosaminyltransferase 1
Beta-1 -3-Galnac-T1
Beta-1 -3-Galactosyltransferase 3
Beta-1 -3-Galtase 3
Beta3gal-T3
Beta3galt3
B3gal-T3
Beta-3-Gx-T3
Galactosylgalactosylglucosylceramide Beta-D-Acetyl-Galactosaminyltransferase
Globoside Synthase
Udp-N-Acetylgalactosamine -Globotriaosylceramide Beta-1 -3-N-Acetylgalactosaminyltransferase
Database Links Reactome: R-HSA-1660662
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Udp-Galnac -Beta-1 -3-N-Acetylgalactosaminyltransferase 1 antibody
Anti-Beta-1 -3-Galnac-T1 antibody
Anti-Beta-1 -3-Galactosyltransferase 3 antibody
Anti-Beta-1 -3-Galtase 3 antibody
Anti-Beta3gal-T3 antibody
Anti-Beta3galt3 antibody
Anti-B3gal-T3 antibody
Anti-Beta-3-Gx-T3 antibody
Anti-Galactosylgalactosylglucosylceramide Beta-D-Acetyl-Galactosaminyltransferase antibody
Anti-Globoside Synthase antibody
Anti-Udp-N-Acetylgalactosamine -Globotriaosylceramide Beta-1 -3-N-Acetylgalactosaminyltransferase antibody
Anti-B3GALNT1 antibody
Anti-B3GALT3 antibody
Anti-UNQ531 antibody
Anti-PRO1074 antibody

Information sourced from Uniprot.org

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