| Host: | Mouse |
| Applications: | WB/IF |
| Reactivity: | Human/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Aurora kinase A is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 2E3-C10-C11-G6 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500 ICC 1:50 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | AURKA |
| Gene ID: | 6790 |
| Uniprot ID: | AURKA_HUMAN |
| Specificity: | This antibody detects endogenous levels of Aurora Kinase A and does not cross-react with related proteins. |
| Immunogen: | Purified recombinant human Aurora Kinase A protein fragments expressed in E.coli. |
| Post Translational Modifications | Activated by phosphorylation at Thr-288.this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Thr-288 can be phosphorylated by several kinases, including PAK and PKA. Protein phosphatase type 1 (PP1) binds AURKA and inhibits its activity by dephosphorylating Thr-288 during mitosis. Phosphorylation at Ser-342 decreases the kinase activity. PPP2CA controls degradation by dephosphorylating Ser-51 at the end of mitosis. Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome. Ubiquitinated by CHFR, leading to its degradation by the proteasome. Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. Ubiquitinated by the CUL3-KLHL18 ligase leading to its activation at the centrosome which is required for initiating mitotic entry. Ubiquitination mediated by CUL3-KLHL18 ligase does not lead to its degradation by the proteasome. |
| Function | Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Important for microtubule formation and/or stabilization. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and destabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Inhibits cilia outgrowth. Required for cilia disassembly via phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin. Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1. |
| Protein Name | Aurora Kinase A Aurora 2 Aurora/Ipl1-Related Kinase 1 Ark-1 Aurora-Related Kinase 1 Breast Tumor-Amplified Kinase Ipl1- And Aurora-Related Kinase 1 Serine/Threonine-Protein Kinase 15 Serine/Threonine-Protein Kinase 6 Serine/Threonine-Protein Kinase Ayk1 Serine/Threonine-Protein Kinase Aurora-A |
| Database Links | Reactome: R-HSA-174178 Reactome: R-HSA-2565942 Reactome: R-HSA-4615885 Reactome: R-HSA-6804114 Reactome: R-HSA-6804756 Reactome: R-HSA-8854050 Reactome: R-HSA-8854518 Reactome: R-HSA-8854521 |
| Cellular Localisation | Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Spindle Pole Centriole Cell Projection Neuron Projection Cilium Cilium Basal Body Basolateral Cell Membrane Detected At The Neurite Hillock In Developing Neurons Localizes At The Centrosome In Mitotic Cells From Early Prophase Until Telophase But Also Localizes To The Spindle Pole Mts From Prophase To Anaphase Colocalized With Sirt2 At Centrosome Moves To The Midbody During Both Telophase And Cytokinesis Associates With Both The Pericentriolar Material (Pcm) And Centrioles The Localization To The Spindle Poles Is Regulated By Aaas |
| Alternative Antibody Names | Anti-Aurora Kinase A antibody Anti-Aurora 2 antibody Anti-Aurora/Ipl1-Related Kinase 1 antibody Anti-Ark-1 antibody Anti-Aurora-Related Kinase 1 antibody Anti-Breast Tumor-Amplified Kinase antibody Anti-Ipl1- And Aurora-Related Kinase 1 antibody Anti-Serine/Threonine-Protein Kinase 15 antibody Anti-Serine/Threonine-Protein Kinase 6 antibody Anti-Serine/Threonine-Protein Kinase Ayk1 antibody Anti-Serine/Threonine-Protein Kinase Aurora-A antibody Anti-AURKA antibody Anti-AIK antibody Anti-AIRK1 antibody Anti-ARK1 antibody Anti-AURA antibody Anti-AYK1 antibody Anti-BTAK antibody Anti-IAK1 antibody Anti-STK15 antibody Anti-STK6 antibody |
Information sourced from Uniprot.org