• Western blot analysis of extracts of various cell lines, using APOL3 antibody (STJ115781) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-APOL3 antibody (1-331) (STJ115781)

SKU:
STJ115781

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOL3 (1-331) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOL3
Gene ID: 80833
Uniprot ID: APOL3_HUMAN
Immunogen Region: 1-331
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human APOL3 (NP_663614.1).
Immunogen Sequence: MDSEKKRFTEEATKYFRERV SPVHLQILLTNNEAWKRFVT AAELPRDEADALYEALKKLR TYAAIEDEYVQQKDEQFREW FLKEFPQVKRKIQESIEKLR ALANGIEEVHRGCTISNVVS SSTGAASGIMSLAGLVLAPF TAGTSLALTAAGVGLGAASA VTGITTSIVEHSYTSSAEAE ASRLTATSIDRLKVFKEVMR DITPNLLSLLNNYYEATQTI GSEIRAIRQARARARLPVT
Tissue Specificity Widely expressed.the highest levels are in prostate, lung and placenta.also detected in kidney, bone marrow, spleen, thymus, spinal cord, adrenal gland, salivary gland, trachea and mammary gland.levels are low in brain, heart, fetal liver, pancreas and testis.
Function May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles.
Protein Name Apolipoprotein L3
Apolipoprotein L-Iii
Apol-Iii
Tnf-Inducible Protein Cg12-1
Cg12_1
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Apolipoprotein L3 antibody
Anti-Apolipoprotein L-Iii antibody
Anti-Apol-Iii antibody
Anti-Tnf-Inducible Protein Cg12-1 antibody
Anti-Cg12_1 antibody
Anti-APOL3 antibody

Information sourced from Uniprot.org

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