Western blot analysis of lysates from mouse spleen, using APOBEC1 Rabbit polyclonal antibody (STJ119163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-APOBEC1 antibody (1-80) (STJ119163)

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STJ119163
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOBEC1
Gene ID: 339
Uniprot ID: ABEC1_HUMAN
Immunogen Region: 1-80
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human APOBEC1 (NP_001291495).
Immunogen Sequence: MTSEKGPSTGDPTLRRRIEP WEFDVFYDPRELRKEACLLY EIKWGMSRKIWRSSGKNTTN HVEVNFIKKFTSERDFHPSM
Tissue Specificity Expressed exclusively in the small intestine.
Function Cytidine deaminase catalyzing the cytidine to uridine postranscriptional editing of a variety of mRNAs. Form complexes with cofactors that confer differential editing activity and selectivity. Responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the apolipoprotein B mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.
Protein Name C->U-Editing Enzyme Apobec-1
Apolipoprotein B Mrna-Editing Enzyme Catalytic Subunit 1
Apo1
Apobec-1
Apolipoprotein B Mrna-Editing Enzyme 1
Hepr
Mrna(Cytosine(6666 Deaminase 1
Database Links Reactome: R-HSA-72200
Reactome: R-HSA-75094
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-C->U-Editing Enzyme Apobec-1 antibody
Anti-Apolipoprotein B Mrna-Editing Enzyme Catalytic Subunit 1 antibody
Anti-Apo1 antibody
Anti-Apobec-1 antibody
Anti-Apolipoprotein B Mrna-Editing Enzyme 1 antibody
Anti-Hepr antibody
Anti-Mrna(Cytosine(6666 Deaminase 1 antibody
Anti-APOBEC1 antibody

Information sourced from Uniprot.org

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