• Western blot analysis of extracts of various cell lines, using ANKS1B antibody (STJ110130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ANKS1B antibody (261-510) (STJ110130)

SKU:
STJ110130

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ANKS1B (261-510) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ANKS1B
Gene ID: 56899
Uniprot ID: ANS1B_HUMAN
Immunogen Region: 261-510
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 261-510 of human ANKS1B (NP_858056.2).
Immunogen Sequence: GFPFSAIHQVHNTGDWGEPS ITLRPPNEATASTPVQYWQH HPEKLIFQSCDYKAFYLGSM LIKELRGTESTQDACAKMRA NCQKSTEQMKKVPTIILSVS YKGVKFIDATNKNIIAEHEI RNISCAAQDPEDLSTFAYIT KDLKSNHHYCHVFTAFDVNL AYEIILTLGQAFEVAYQLAL QARKGGHSSTLPESFENKPS KPIPKPRVSIRKSVQIDPSE QKTLANLPWIVEPGQEAKR
Tissue Specificity Highly expressed in marrow from patients with pre-B ALL associated with the t(1.9) translocation. Strongly expressed in brain and testis. Expressed in fetal brain. Isoform 4 is highly expressed in brain (at protein level). Isoform 6 is expressed in brain and several cancer cell lines.
Post Translational Modifications Isoform 3 nuclear translocation requires an NMDAR-dependent proteolytic cleavage.
Function Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells. Isoform 3 can regulate global protein synthesis by altering nucleolar numbers. Isoform 4 may play a role as a modulator of APP processing. Overexpression can down-regulate APP processing.
Protein Name Ankyrin Repeat And Sterile Alpha Motif Domain-Containing Protein 1b
Amyloid-Beta Protein Intracellular Domain-Associated Protein 1
Aida-1
E2a-Pbx1-Associated Protein
Eb-1
Cellular Localisation Cytoplasm
Isoform 2: Nucleus
Isoform 3: Postsynaptic Density
Cell Projection
Dendritic Spine
Nucleus
Cajal Body
The Synaptic Localization Requires Dlg4 Interaction
Translocation To The Nucleus In Response To Stimulation Of Nmda Receptors (Nmdars) In A Calcium-Independent Manner
Isoform 4: Nucleus
The Interaction With App Causes Its Partial Exclusion From The Nucleus
When App Is Overexpressed
Isoform 6: Nucleus
Alternative Antibody Names Anti-Ankyrin Repeat And Sterile Alpha Motif Domain-Containing Protein 1b antibody
Anti-Amyloid-Beta Protein Intracellular Domain-Associated Protein 1 antibody
Anti-Aida-1 antibody
Anti-E2a-Pbx1-Associated Protein antibody
Anti-Eb-1 antibody
Anti-ANKS1B antibody

Information sourced from Uniprot.org

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