• Western blot analysis of lysates from 1) Jurkat, 2) K562, 3) HuvEC cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • Western blot analysis of various cells using Alpha-SMA Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000

Anti-ACTA2 antibody (84-134 aa) (STJ96418)

SKU:
STJ96418

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Actin, aortic smooth muscle (84-134 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTA2
Gene ID: 59
Uniprot ID: ACTA_HUMAN
Immunogen Region: 84-134 aa
Specificity: Alpha-SMA Polyclonal Antibody detects endogenous levels of Alpha-SMA protein.
Immunogen: Synthesized peptide derived from the N-terminal region of human Alpha-SMA at the amino acid range 84-134
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Protein Name Actin - Aortic Smooth Muscle
Alpha-Actin-2
Cell Growth-Inhibiting Gene 46 Protein Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form
Database Links Reactome: R-HSA-445355
Reactome: R-HSA-9013695
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Actin - Aortic Smooth Muscle antibody
Anti-Alpha-Actin-2 antibody
Anti-Cell Growth-Inhibiting Gene 46 Protein Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form antibody
Anti-ACTA2 antibody
Anti-ACTSA antibody
Anti-ACTVS antibody
Anti-GIG46 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance