• Western blot analysis of various lysates using ALG9 Rabbit polyclonal antibody (STJ119346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ALG9 antibody (439-618) (STJ119346)

SKU:
STJ119346

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALG9
Gene ID: 79796
Uniprot ID: ALG9_HUMAN
Immunogen Region: 439-618
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 439-618 of human ALG9 (NP_079016.2).
Immunogen Sequence: LFRGYHGPLDLYPEFYRIAT DPTIHTVPEGRPVNVCVGKE WYRFPSSFLLPDNWQLQFIP SEFRGQLPKPFAEGPLATRI VPTDMNDQNLEEPSRYIDIS KCHYLVDLDTMRETPREPKY SSNKEEWISLAYRPFLDASR SSKLLRAFYVPFLSDQYTVY VNYTILKPRKAKQIRKKSGG
Tissue Specificity Ubiquitously expressed.with highest levels in heart, liver and pancreas.
Function Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, catalyzes the addition of the seventh and ninth alpha-1,2-linked mannose residues to Man(6)GlcNAc(2)-PP-dolichol and Man(8)GlcNAc(2)-PP-dolichol respectively.
Protein Name Alpha-1 -2-Mannosyltransferase Alg9
Asparagine-Linked Glycosylation Protein 9 Homolog
Disrupted In Bipolar Disorder Protein 1
Dol-P-Man -Man(6glcnac(2-Pp-Dol Alpha-1 -2-Mannosyltransferase
Dol-P-Man -Man(8glcnac(2-Pp-Dol Alpha-1 -2-Mannosyltransferase
Database Links Reactome: R-HSA-446193
Reactome: R-HSA-4720454
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Alpha-1 -2-Mannosyltransferase Alg9 antibody
Anti-Asparagine-Linked Glycosylation Protein 9 Homolog antibody
Anti-Disrupted In Bipolar Disorder Protein 1 antibody
Anti-Dol-P-Man -Man(6glcnac(2-Pp-Dol Alpha-1 -2-Mannosyltransferase antibody
Anti-Dol-P-Man -Man(8glcnac(2-Pp-Dol Alpha-1 -2-Mannosyltransferase antibody
Anti-ALG9 antibody
Anti-DIBD1 antibody

Information sourced from Uniprot.org

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