| Host: | Goat |
| Applications: | Pep-ELISA |
| Reactivity: | Human/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-AKAP9/YOTIAO (N-Term) is suitable for use in ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000. WB: Not yet tested-our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 453kDa according to NP_005742.4. Therefore we cannot |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | AKAP9 |
| Gene ID: | 10142 |
| Uniprot ID: | AKAP9_HUMAN |
| Immunogen Region: | N-Term |
| Accession Number: | NP_005742.4; NP_671714.1 |
| Specificity: | This antibody is expected to recognize isoforms 2 and 3 (NP_005742.4; NP_671714.1) |
| Immunogen Sequence: | QRKAQSDGQSPSKK |
| Function | Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus. Required for microtubule nucleation at the cis-side of the Golgi apparatus. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. In complex with PDE4DIP isoform 13/MMG8/SMYLE, recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. In complex with PDE4DIP isoform 13/MMG8/SMYLE, EB1/MAPRE1 and CDK5RAP2, contributes to microtubules nucleation and extension also from the centrosome to the cell periphery. Isoform 4: Associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations. |
| Protein Name | A-Kinase Anchor Protein 9 Akap-9 A-Kinase Anchor Protein 350 Kda Akap 350 Hgakap 350 A-Kinase Anchor Protein 450 Kda Akap 450 Akap 120-Like Protein Centrosome- And Golgi-Localized Pkn-Associated Protein Cg-Nap Protein Hyperion Protein Kinase A-Anchoring Protein 9 Prka9 Protein Yotiao |
| Database Links | Reactome: R-HSA-2565942 Reactome: R-HSA-380259 Reactome: R-HSA-380270 Reactome: R-HSA-380284 Reactome: R-HSA-380320 Reactome: R-HSA-5576890 Reactome: R-HSA-5576893 Reactome: R-HSA-5620912 Reactome: R-HSA-6802952 Reactome: R-HSA-8854518 |
| Cellular Localisation | Golgi Apparatus Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Cytoplasmic In Parietal Cells Recruited To The Golgi Apparatus By Gm130/Golga2 Localization At The Centrosome Versus Golgi Apparatus May Be Cell Line-Dependent In Skbr3 And Hek293f Cells Exclusively Located At The Centrosome In Hela Mda-Mb231 And Rpe-1 Cells Detected At The Golgi Apparatus In Sk-Br-3 Cells Recruited To The Centrosome In The Presence Of Cdk5rap2 |
| Alternative Antibody Names | Anti-A-Kinase Anchor Protein 9 antibody Anti-Akap-9 antibody Anti-A-Kinase Anchor Protein 350 Kda antibody Anti-Akap 350 antibody Anti-Hgakap 350 antibody Anti-A-Kinase Anchor Protein 450 Kda antibody Anti-Akap 450 antibody Anti-Akap 120-Like Protein antibody Anti-Centrosome- And Golgi-Localized Pkn-Associated Protein antibody Anti-Cg-Nap antibody Anti-Protein Hyperion antibody Anti-Protein Kinase A-Anchoring Protein 9 antibody Anti-Prka9 antibody Anti-Protein Yotiao antibody Anti-AKAP9 antibody Anti-AKAP350 antibody Anti-AKAP450 antibody Anti-KIAA0803 antibody |
Information sourced from Uniprot.org