||IF, WB, IHC-P
||Human, Mouse, Rat, Chicken
||Mouse monoclonal anti-Active Caspase-3 antibody is suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
||FOR RESEARCH USE ONLY (RUO).
||PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide and 50% Glycerol.
||The antibody was affinity-purified from mouse ascites by affinity-chromatography using a epitope-specific immunogen.
||Store at-20°C, and avoid repeat freeze-thaw cycles.
||IF 1:50-200WB 1:500-1000IHC 1:100-200
||The antibody detects endogenous Active Caspase-3 protein.
| Post Translational Modifications || Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. |
| Function || Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. |
| Protein Name || Caspase-3Casp-3ApopainCysteine Protease Cpp32Cpp-32Protein YamaSrebp Cleavage Activity 1Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12 |
| Database Links || Reactome: R-HSA-111459Reactome: R-HSA-111463Reactome: R-HSA-111464Reactome: R-HSA-111465Reactome: R-HSA-111469Reactome: R-HSA-140342Reactome: R-HSA-1474228Reactome: R-HSA-2028269Reactome: R-HSA-205025Reactome: R-HSA-211736Reactome: R-HSA-264870Reactome: R-HSA-351906Reactome: R-HSA-418889Reactome: R-HSA-449836 |
| Cellular Localisation || Cytoplasm |
| Alternative Antibody Names || Anti-Caspase-3 antibodyAnti-Casp-3 antibodyAnti-Apopain antibodyAnti-Cysteine Protease Cpp32 antibodyAnti-Cpp-32 antibodyAnti-Protein Yama antibodyAnti-Srebp Cleavage Activity 1 antibodyAnti-Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12 antibodyAnti-CASP3 antibodyAnti-CPP32 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance