• Western blot analysis of chicken cell lysis using Antibody diluted at 1:1000
  • Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue using Active Caspase-3 monoclonal antibody.
  • Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using Active Caspase-3 monoclonal antibody.
  • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Active Caspase-3 monoclonal antibody (5E1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Active Caspase-3 monoclonal antibody (5E1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Active Caspase-3 monoclonal antibody (5E1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of rat-lung tissue. 1, Active Caspase-3 monoclonal antibody (5E1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Hela cell. 1, FoxO1 (phospho Ser256) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Active Caspase-3 antibody [5E1] (STJ97448)

SKU:
STJ97448

Current Stock:
Host: Mouse
Applications: IF, WB, IHC-P
Reactivity: Human, Mouse, Rat, Chicken
Short Description: Mouse monoclonal anti-Active Caspase-3 antibody is suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: 5E1
Conjugation: Unconjugated
Formulation: PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/mL
Isotype: IgG1
Dilution Range: IF 1:50-200
WB 1:500-1000
IHC 1:100-200
Specificity: The antibody detects endogenous Active Caspase-3 protein.
Uniprot ID: CASP3_HUMAN
Gene ID: 836
Gene Symbol: CASP3
Immunogen: Recombinant Protein
Post Translational Modifications Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Protein Name Caspase-3
Casp-3
Apopain
Cysteine Protease Cpp32
Cpp-32
Protein Yama
Srebp Cleavage Activity 1
Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12
Database Links Reactome: R-HSA-111459
Reactome: R-HSA-111463
Reactome: R-HSA-111464
Reactome: R-HSA-111465
Reactome: R-HSA-111469
Reactome: R-HSA-140342
Reactome: R-HSA-1474228
Reactome: R-HSA-2028269
Reactome: R-HSA-205025
Reactome: R-HSA-211736
Reactome: R-HSA-264870
Reactome: R-HSA-351906
Reactome: R-HSA-418889
Reactome: R-HSA-449836
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Caspase-3 antibody
Anti-Casp-3 antibody
Anti-Apopain antibody
Anti-Cysteine Protease Cpp32 antibody
Anti-Cpp-32 antibody
Anti-Protein Yama antibody
Anti-Srebp Cleavage Activity 1 antibody
Anti-Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12 antibody
Anti-CASP3 antibody
Anti-CPP32 antibody

Information sourced from Uniprot.org

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