• Western blot analysis of 1, mouse-liver 2, hela cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-Acetyl-E2F1-K125 antibody (K125) (STJ98840)

SKU:
STJ98840

Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-Acetyl-Transcription Factor E2f1-K125 (K125) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: E2F1
Gene ID: 1869
Uniprot ID: E2F1_HUMAN
Immunogen Region: K125
Specificity: Acetyl-E2F1-K125 polyclonal antibody (Transcription Factor E2f1) binds to endogenous Transcription Factor E2f1 at the amino acid region K125.
Immunogen: Synthesized Acetyl peptide derived from human E2F-1. at amino acid range: K125
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Positively regulates transcription of RRP1B.
Protein Name Transcription Factor E2f1
E2f-1
Pbr3
Retinoblastoma-Associated Protein 1
Rbap-1
Retinoblastoma-Binding Protein 3
Rbbp-3
Prb-Binding Protein E2f-1
Database Links Reactome: R-HSA-111448
Reactome: R-HSA-113501
Reactome: R-HSA-1362277
Reactome: R-HSA-1362300
Reactome: R-HSA-139915
Reactome: R-HSA-1912408
Reactome: R-HSA-2559580
Reactome: R-HSA-2559585
Reactome: R-HSA-6804116
Reactome: R-HSA-68689
Reactome: R-HSA-68911
Reactome: R-HSA-69202
Reactome: R-HSA-69205
Reactome: R-HSA-69231
Reactome: R-HSA-69656
Reactome: R-HSA-8953750
Reactome: R-HSA-9616222
Reactome: R-HSA-9661069
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor E2f1 antibody
Anti-E2f-1 antibody
Anti-Pbr3 antibody
Anti-Retinoblastoma-Associated Protein 1 antibody
Anti-Rbap-1 antibody
Anti-Retinoblastoma-Binding Protein 3 antibody
Anti-Rbbp-3 antibody
Anti-Prb-Binding Protein E2f-1 antibody
Anti-E2F1 antibody
Anti-RBBP3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance