| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ABAD/HADH2 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 25kDa band observed in Human Brain lysates (calculated MW of 26.9kDa according to NP_004484). Recommended concentration: 0.3-2µg/ml. Primary incubation 1 hour at room temperatur |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | HSD17B10 |
| Gene ID: | 3028 |
| Uniprot ID: | HCD2_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_004484.1; NP_001032900.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms. |
| Immunogen Sequence: | CIRLDGAIRMQP |
| Function | Mitochondrial dehydrogenase involved in pathways of fatty acid, branched-chain amino acid and steroid metabolism. Acts as (S)-3-hydroxyacyl-CoA dehydrogenase in mitochondrial fatty acid beta-oxidation, a major degradation pathway of fatty acids. Catalyzes the third step in the beta-oxidation cycle, namely the reversible conversion of (S)-3-hydroxyacyl-CoA to 3-ketoacyl-CoA. Preferentially accepts straight medium- and short-chain acyl-CoA substrates with highest efficiency for (3S)-hydroxybutanoyl-CoA. Acts as 3-hydroxy-2-methylbutyryl-CoA dehydrogenase in branched-chain amino acid catabolic pathway. Catalyzes the oxidation of 3-hydroxy-2-methylbutanoyl-CoA into 2-methyl-3-oxobutanoyl-CoA, a step in isoleucine degradation pathway. Has hydroxysteroid dehydrogenase activity toward steroid hormones and bile acids. Catalyzes the oxidation of 3alpha-, 17beta-, 20beta- and 21-hydroxysteroids and 7alpha- and 7beta-hydroxy bile acids. Oxidizes allopregnanolone/brexanolone at the 3alpha-hydroxyl group, which is known to be critical for the activation of gamma-aminobutyric acid receptors (GABAARs) chloride channel. Has phospholipase C-like activity toward cardiolipin and its oxidized species. Likely oxidizes the 2'-hydroxyl in the head group of cardiolipin to form a ketone intermediate that undergoes nucleophilic attack by water and fragments into diacylglycerol, dihydroxyacetone and orthophosphate. Has higher affinity for cardiolipin with oxidized fatty acids and may degrade these species during the oxidative stress response to protect cells from apoptosis. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD). Essential for structural and functional integrity of mitochondria. In addition to mitochondrial dehydrogenase activity, moonlights as a component of mitochondrial ribonuclease P, a complex that cleaves tRNA molecules in their 5'-ends. Together with TRMT10C/MRPP1, forms a subcomplex of the mitochondrial ribonuclease P, named MRPP1-MRPP2 subcomplex, which displays functions that are independent of the ribonuclease P activity. The MRPP1-MRPP2 subcomplex catalyzes the formation of N(1)-methylguanine and N(1)-methyladenine at position 9 (m1G9 and m1A9, respectively) in tRNAs.HSD17B10/MRPP2 acting as a non-catalytic subunit. The MRPP1-MRPP2 subcomplex also acts as a tRNA maturation platform: following 5'-end cleavage by the mitochondrial ribonuclease P complex, the MRPP1-MRPP2 subcomplex enhances the efficiency of 3'-processing catalyzed by ELAC2, retains the tRNA product after ELAC2 processing and presents the nascent tRNA to the mitochondrial CCA tRNA nucleotidyltransferase TRNT1 enzyme. Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly. |
| Protein Name | 3-Hydroxyacyl-Coa Dehydrogenase Type-2 17-Beta-Estradiol 17-Dehydrogenase 2-Methyl-3-Hydroxybutyryl-Coa Dehydrogenase Mhbd 3-Alpha-(17-Beta-Hydroxysteroid Dehydrogenase Nad(+ 3-Hydroxy-2-Methylbutyryl-Coa Dehydrogenase 3-Hydroxyacyl-Coa Dehydrogenase Type Ii 3alpha(Or 20beta-Hydroxysteroid Dehydrogenase 7-Alpha-Hydroxysteroid Dehydrogenase Endoplasmic Reticulum-Associated Amyloid Beta-Peptide-Binding Protein Mitochondrial Ribonuclease P Protein 2 Mitochondrial Rnase P Protein 2 Short Chain Dehydrogenase/Reductase Family 5c Member 1 Short-Chain Type Dehydrogenase/Reductase Xh98g2 Type Ii Hadh |
| Database Links | Reactome: R-HSA-6785470 Reactome: R-HSA-6787450 Reactome: R-HSA-70895 Reactome: R-HSA-8868766 Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion Mitochondrion Matrix Mitochondrion Nucleoid |
| Alternative Antibody Names | Anti-3-Hydroxyacyl-Coa Dehydrogenase Type-2 antibody Anti-17-Beta-Estradiol 17-Dehydrogenase antibody Anti-2-Methyl-3-Hydroxybutyryl-Coa Dehydrogenase antibody Anti-Mhbd antibody Anti-3-Alpha-(17-Beta-Hydroxysteroid Dehydrogenase antibody Anti-Nad(+ antibody Anti-3-Hydroxy-2-Methylbutyryl-Coa Dehydrogenase antibody Anti-3-Hydroxyacyl-Coa Dehydrogenase Type Ii antibody Anti-3alpha(Or 20beta-Hydroxysteroid Dehydrogenase antibody Anti-7-Alpha-Hydroxysteroid Dehydrogenase antibody Anti-Endoplasmic Reticulum-Associated Amyloid Beta-Peptide-Binding Protein antibody Anti-Mitochondrial Ribonuclease P Protein 2 antibody Anti-Mitochondrial Rnase P Protein 2 antibody Anti-Short Chain Dehydrogenase/Reductase Family 5c Member 1 antibody Anti-Short-Chain Type Dehydrogenase/Reductase Xh98g2 antibody Anti-Type Ii Hadh antibody Anti-HSD17B10 antibody Anti-ERAB antibody Anti-HADH2 antibody Anti-MRPP2 antibody Anti-SCHAD antibody Anti-SDR5C1 antibody Anti-XH98G2 antibody |
Information sourced from Uniprot.org
