• Western blot analysis of lysates from RAW264.7 cells, using TNAP3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of 293 cells using A20 Polyclonal Antibody
  • Immunofluorescence analysis of HepG2 cells, using TNAP3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using A20 Polyclonal Antibody

Anti-A20 antibody (STJ91402)

Current Stock:
Host: Rabbit
Applications: WB, IF, ELISA
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal primary antibody against A20.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Specificity: A20 polyclonal antibody detects endogenous levels of A20 protein.
Gene ID: 7128
Gene Symbol: TNFAIP3
Immunogen: Synthesized peptide derived from human A20
Immunogen Region: 290-370 aa, Internal
Post Translational Modifications Proteolytically cleaved by MALT1 upon TCR stimulation.disrupts NF-kappa-B inhibitory function and results in increased IL-2 production. It is proposed that only a fraction of TNFAIP3 colocalized with TCR and CBM complex is cleaved, leaving the main TNFAIP3 pool intact.
Function Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways.affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG.the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin.polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.
Database Links Reactome: R-HSA-168638
Cellular Localisation Cytoplasm
A20p50: Cytoplasm
Protein Name Tumor Necrosis Factor Alpha-Induced Protein 3
Tnf Alpha-Induced Protein 3
Otu Domain-Containing Protein 7c
Putative Dna-Binding Protein A20
Zinc Finger Protein A20 Cleaved Into - A20p50 - A20p37
Alternative Names Anti-Tumor Necrosis Factor Alpha-Induced Protein 3 antibody
Anti-Tnf Alpha-Induced Protein 3 antibody
Anti-Otu Domain-Containing Protein 7c antibody
Anti-Putative Dna-Binding Protein A20 antibody
Anti-Zinc Finger Protein A20 Cleaved Into - A20p50 - A20p37 antibody
Anti-TNFAIP3 antibody
Anti-OTUD7C antibody

Information sourced from Uniprot.org

6 months standard