• Immunohistochemistry analysis of A1CF in paraffin-embedded mouse brain using A1CF Rabbit polyclonal antibody (STJ115054) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of A1CF in paraffin-embedded human tonsil using A1CF Rabbit polyclonal antibody (STJ115054) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of A1CF in paraffin-embedded rat ovary using A1CF Rabbit polyclonal antibody (STJ115054) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HepG2 cells, using A1CF Rabbit polyclonal antibody (STJ115054) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-A1CF antibody (50-120) (STJ115054)

SKU:
STJ115054

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-A1CF (50-120) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: A1CF
Gene ID: 29974
Uniprot ID: A1CF_HUMAN
Immunogen Region: 50-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-120 of human A1CF (NP_055391.2).
Immunogen Sequence: APPERGCEIFIGKLPRDLFE DELIPLCEKIGKIYEMRMMM DFNGNNRGYAFVTFSNKVEA KNAIKQLNNYE
Tissue Specificity Widely expressed with highest levels in brain, liver, pancreas, colon and spleen.
Function Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.
Protein Name Apobec1 Complementation Factor
Apobec1-Stimulating Protein
Database Links Reactome: R-HSA-72200
Reactome: R-HSA-75094
Cellular Localisation Nucleus
Endoplasmic Reticulum
Cytoplasm
Predominantly Nuclear Where It Localizes To Heterochromatin
Also Cytoplasmic Where It Is Found At The Outer Surface Of The Endoplasmic Reticulum
Shuttles Between The Nucleus And Cytoplasm
May Be Transported Into The Nucleus By The Nuclear Import Protein Tnpo2/Trn2 Or By Apobec1
Alternative Antibody Names Anti-Apobec1 Complementation Factor antibody
Anti-Apobec1-Stimulating Protein antibody
Anti-A1CF antibody
Anti-ACF antibody
Anti-ASP antibody

Information sourced from Uniprot.org

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