• Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using MAPK3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from COLO205 cells, using MAPK3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using 3pK Polyclonal Antibody

Anti-3pK antibody (STJ91379)

SKU:
STJ91379

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-3pK antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Specificity: 3pK polyclonal antibody detects endogenous levels of 3pK protein.
Uniprot ID: MAPK3_HUMAN
Gene ID: 7867
Gene Symbol: MAPKAPK3
Immunogen: Synthesized peptide derived from human 3pK
Immunogen Region: 270-350 aa, C-terminal
Post Translational Modifications Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313.
Function Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
Protein Name Map Kinase-Activated Protein Kinase 3
Mapk-Activated Protein Kinase 3
Mapkap Kinase 3
Mapkap-K3
Mapkapk-3
Mk-3
Chromosome 3p Kinase
3pk
Database Links Reactome: R-HSA-171007
Reactome: R-HSA-2559580
Reactome: R-HSA-4420097
Reactome: R-HSA-450302
Cellular Localisation Nucleus
Cytoplasm
Predominantly Located In The Nucleus
When Activated It Translocates To The Cytoplasm
Alternative Antibody Names Anti-Map Kinase-Activated Protein Kinase 3 antibody
Anti-Mapk-Activated Protein Kinase 3 antibody
Anti-Mapkap Kinase 3 antibody
Anti-Mapkap-K3 antibody
Anti-Mapkapk-3 antibody
Anti-Mk-3 antibody
Anti-Chromosome 3p Kinase antibody
Anti-3pk antibody
Anti-MAPKAPK3 antibody

Information sourced from Uniprot.org

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