Tissue Specificity | Detected in blood plasma (at protein level). |
Function | Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues. Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane. |
Protein Name | Retinol-Binding Protein 4Plasma Retinol-Binding ProteinPrbpRbp |
Database Links | Reactome: R-RNO-2453902Reactome: -RNO-975634 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Retinol-Binding Protein 4 ELISA kitPlasma Retinol-Binding Protein ELISA kitPrbp ELISA kitRbp ELISA kitRbp4 ELISA kit |
Specificity | This kit recognizes Rat RBP4 in samples. No significant cross-reactivity or interference between Rat RBP4 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 87-100 | 94 |
EDTA plasma (n=8) | 94-106 | 100 |
Cell culture media (n=8) | 93-107 | 100 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (μg/mL) | 1.10 | 2.93 | 7.64 | 1.14 | 3.15 | 6.98 |
Standard deviation | 0.07 | 0.12 | 0.41 | 0.06 | 0.13 | 0.21 |
CV (%) | 6.32 | 4.02 | 5.40 | 5.61 | 4.19 | 3.08 |