| Applications: | ELISA |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.27ng/mL |
| Detection Limit: | 0.781-50ng/mL |
| Short Description : | This HSPA5 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | Hspa5 |
| Gene ID: | 25617 |
| Uniprot ID: | BIP_RAT |
| Sample Type: | tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | In unstressed cells, AMPylation at Thr-518 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins. In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity. |
| Function | Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the EIF2AK3/PERK and ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Also binds and inactivates EIF2AK3/PERK in unstressed cells. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1 and EIF2AK3/PERK, allowing their homodimerization and subsequent activation. Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation. |
| Protein Name | Endoplasmic Reticulum Chaperone Bip 78 Kda Glucose-Regulated Protein Grp-78 Binding-Immunoglobulin Protein Bip Heat Shock Protein 70 Family Protein 5 Hsp70 Family Protein 5 Heat Shock Protein Family A Member 5 Immunoglobulin Heavy Chain-Binding Protein Steroidogenesis-Activator Polypeptide |
| Database Links | Reactome: R-RNO-3371453 Reactome: -RNO-983170 |
| Cellular Localisation | Endoplasmic Reticulum Lumen Melanosome Cytoplasm Cell Surface Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv Localizes To The Cell Surface In Epithelial Cells High Levels Of Free Iron Promotes Cell Surface Localization |
| Alternative ELISA Names | Endoplasmic Reticulum Chaperone Bip ELISA kit 78 Kda Glucose-Regulated Protein ELISA kit Grp-78 ELISA kit Binding-Immunoglobulin Protein ELISA kit Bip ELISA kit Heat Shock Protein 70 Family Protein 5 ELISA kit Hsp70 Family Protein 5 ELISA kit Heat Shock Protein Family A Member 5 ELISA kit Immunoglobulin Heavy Chain-Binding Protein ELISA kit Steroidogenesis-Activator Polypeptide ELISA kit Hspa5 ELISA kit Grp78 ELISA kit |
| output |
Information sourced from Uniprot.org