| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
30.51 pg/mL |
| Detection Limit: |
78.13-5000 pg/mL |
| Short Description: |
This FETUA Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Ahsg |
| Gene ID: |
25373 |
| Uniprot ID: |
FETUA_RAT |
| Specificity: |
This kit recognizes Rat FETUA in samples. No significant cross-reactivity or interference between Rat FETUA and analogues was observed. |
| Sample Type: |
serum, plasma and other biological fluids |
| Tissue Specificity | Synthesized in liver and secreted by the hepatocytes in the blood. |
| Post Translational Modifications | Undergoes complex post-translational modification involving N-glycosylation, and addition of fucose and sialic acid residues. Phosphorylation occurs at a serine residue. Phosphorylated by FAM20C in the extracellular medium. |
| Function | Could inhibit both insulin-receptor tyrosine kinase activity and insulin-stimulated receptor autophosphorylation and, concomitantly, antagonize the mitogenic effect of the hormone in cultured rat hepatoma cells. |
| Protein Name | Alpha-2-Hs-Glycoprotein59 Kda Bone Sialic Acid-Containing ProteinBspFetuin-AGlycoprotein Pp63 |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-381426Reactome: -RNO-6798695Reactome: -RNO-8957275 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Alpha-2-Hs-Glycoprotein ELISA kit59 Kda Bone Sialic Acid-Containing Protein ELISA kitBsp ELISA kitFetuin-A ELISA kitGlycoprotein Pp63 ELISA kitAhsg ELISA kitFetua ELISA kit |
| Specificity | This kit recognizes Rat FETUA in samples. No significant cross-reactivity or interference between Rat FETUA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-110 | 102 |
| EDTA plasma (n=8) | 88-101 | 94 |
| Cell culture media (n=8) | 94-107 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 276.77 | 575.17 | 2244.01 | 298.37 | 519.68 | 2172.91 |
| Standard deviation | 17.44 | 25.65 | 70.01 | 15.96 | 21.41 | 107.99 |
| CV (%) | 6.30 | 4.46 | 3.12 | 5.35 | 4.12 | 4.97 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
