Mouse IL-6 protein (Recombinant) (C-His) (STJP001314)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse IL-6 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe25-Thr211) of Mouse IL6 (Accession #NP_112445.1) fused with an 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Mouse IL-6-C-His protein was developed in hek293 cells using the region Phe25-Thr211. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.07-0.28 ng/mL, corresponding to a specific activity of 3.57×106 ~ 1.43×107 units/mg. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Il6 |
| Gene ID | 16193 |
| Uniprot ID | IL6_MOUSE |
| Immunogen | Recombinant Mouse IL-6 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe25-Thr211) of Mouse IL6 (Accession #NP_112445.1) fused with an 6×His tag at the C-terminus. |
| Immunogen Region | Phe25-Thr211 |
| Immunogen Sequence | FPTSQVRRGDFTEDTTPNRP VYTTSQVGGLITHVLWEIVE MRKELCNGNSDCMNNDDALA ENNLKLPEIQRNDGCYQTG YNQEICLLKISSGLLEYHSY LEYMKNNLKDNKKDKARVLQ R DTETLIHIFNQEVKDLHK IVLPTPISNALLTDKLESQK EWLRTKTIQFILKSLEEFLK VT LRSTRQT |
Additional Info
| Tissue Specificity | Expressed by dendritic cells and macrophages. Expressed by activated follicular B cells. Abundantly expressed in the central nervous system (CNS), particularly the hypothalamic region. |
| Post Translational Modifications | N- and O-glycosylated. |
| Function | Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism (Probable). Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells. IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B-cells into immunoglolin-secreting cells (Probable). Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Together with IL21, controls the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. Required to drive naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells. Also required for proliferation of myeloma cells and the survival of plasmablast cells (Probable). Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration. |
| Protein Name | Interleukin-6Il-6B-Cell Hybridoma Growth FactorInterleukin Hp-1 |
| Database Links | Reactome: R-MMU-1059683Reactome: -MMU-110056Reactome: -MMU-112411Reactome: -MMU-381426Reactome: -MMU-8957275 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-6 proteinIl-6 proteinB-Cell Hybridoma Growth Factor proteinInterleukin Hp-1 proteinIl6 proteinIl-6 protein |
Information sourced from Uniprot.org