| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.049ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description : | This H4 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma, tissue homogenates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | H4c1.H4c2.H4c3.H4c4.H4c6.H4c8.H4c9.H4c11.H4c12.Hist1h4m.H4c14.H4c16 |
| Gene ID: | 100041230/319155/319156/319157/319158/319159/319160/319161/320332/326619/326620/69386/97122 |
| Uniprot ID: | H4_MOUSE |
| Sample Type: | serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by KMT5A/SET8. Trimethylation is performed by KMT5B and KMT5C and induces gene silencing. Monomethylated at Lys-13 (H4K12me1) by N6AMT1.H4K12me1 modification is present at the promoters of numerous genes encoding cell cycle regulators. Acetyl-methylated at Lys-6 and Lys-13 (H4K5acme and H4K12acme, respectively), acetyl-methylation is an epigenetic mark of active chromatin associated with increased transcriptional initiation. Acetyl-methylation is formed by acetylation by EP300/p300 of lysine residues that are already monomethylated on the same side chain. H4K5acme and H4K12acme marks specifically bind BRD2. Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Sumoylated, which is associated with transcriptional repression. Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis. Hydroxybutyrylation of histones is induced by starvation. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Glutarylation at Lys-92 (H4K91glu) destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes. Ufmylated.monofmylated by UFL1 at Lys-32 (H4K31Ufm1) in response to DNA damage. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription. Delactylated by SIRT3 at Lys-17 (H4K16la). |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Protein Name | Histone H4 |
| Database Links | Reactome: R-MMU-110330 Reactome: -MMU-110331 Reactome: -MMU-212300 Reactome: -MMU-2299718 Reactome: -MMU-2559586 Reactome: -MMU-3214815 Reactome: -MMU-3214841 Reactome: -MMU-3214842 Reactome: -MMU-3214847 Reactome: -MMU-3214858 Reactome: -MMU-4551638 Reactome: -MMU-5693565 Reactome: -MMU-5693571 Reactome: -MMU-5693607 Reactome: -MMU-606279 Reactome: -MMU-69473 Reactome: -MMU-8936459 Reactome: -MMU-9018519 Reactome: -MMU-9670095 Reactome: -MMU-9843940 |
| Cellular Localisation | Nucleus Chromosome |
| Alternative ELISA Names | Histone H4 ELISA kit H4c1 ELISA kit Hist1h4a.H4c2 ELISA kit H4-53 ELISA kit Hist1h4b.H4c3 ELISA kit H4-12 ELISA kit Hist1h4c.H4c4 ELISA kit Hist1h4d.H4c6 ELISA kit Hist1h4f.H4c8 ELISA kit Hist1h4h.H4c9 ELISA kit Hist1h4i.H4c11 ELISA kit Hist1h4j.H4c12 ELISA kit Hist1h4k.Hist1h4m.H4c14 ELISA kit Hist2h4 ELISA kit Hist2h4a.H4c16 ELISA kit H4f16 ELISA kit Hist4h4 ELISA kit |
| output |
Information sourced from Uniprot.org