Lin28 Blocking Peptide peptide (STJ504301)

SKU:
STJ504301-250

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Applications: Immunodepletion/Immunocompetition
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Lin28 Blocking Peptide is synthetically produced from the 1-60 aa sequence and is suitable for use in western blot applications.
Formulation: Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: LIN28A
Gene ID: 79727
Uniprot ID: LN28A_HUMAN
Immunogen Region: 1-60 aa
Immunogen: Synthetic peptide taken within amino acid sequence 1-60 on Lin28A sequence.
Tissue Specificity Expressed in embryonic stem cells, placenta and testis. Tends to be up-regulated in HER2-overexpressing breast tumors.
Function RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 and TUT7 uridylyltransferases. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells. Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue.
Peptide Name Protein Lin-28 Homolog A
Lin-28a
Zinc Finger Cchc Domain-Containing Protein 1
Database Links Reactome: R-HSA-452723
Cellular Localisation Cytoplasm
Rough Endoplasmic Reticulum
P-Body
Stress Granule
Nucleus
Nucleolus
Predominantly Cytoplasmic
In The Cytoplasm
Localizes To Peri-Endoplasmic Reticulum Regions And Detected In The Microsomal Fraction Derived From Rough Endoplasmic Reticulum (Rer) Following Subcellular Fractionation
May Be Bound To The Cytosolic Surface Of Rer On Which Er-Associated Mrnas Are Translated
Shuttle From The Nucleus To The Cytoplasm Requires Rna-Binding
Nucleolar Localization Is Observed In 10-15% Of The Nuclei In Differentiated Myotubes
Alternative Peptide Names Protein Lin-28 Homolog A protein
Lin-28a protein
Zinc Finger Cchc Domain-Containing Protein 1 protein
LIN28A protein
CSDD1 protein
LIN28 protein
ZCCHC1 protein

Information sourced from Uniprot.org

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