| Applications: | CLIA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Short Description: | This human NRG-1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | NRG1 |
| Gene ID: | 3084 |
| Uniprot ID: | NRG1_HUMAN |
| Specificity: | This kit recognizes Human NRG-1 in samples. No significant cross-reactivity or interference between Human NRG-1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells.in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. |
| Post Translational Modifications | Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage. |
| Function | Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells.inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction.stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells.stimulating Schwann cell proliferation.implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4. Binds to ERBB3. Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons. |
| Protein Name | Pro-Neuregulin-1 - Membrane-Bound IsoformPro-Nrg1 Cleaved Into - Neuregulin-1Acetylcholine Receptor-Inducing ActivityAriaBreast Cancer Cell Differentiation Factor P45Glial Growth FactorHeregulinHrgNeu Differentiation FactorSensory And Motor Neuron-Derived Factor |
| Database Links | Reactome: R-HSA-1227986 Q02297-10Reactome: R-HSA-1236394 Q02297-10Reactome: R-HSA-1250196 Q02297-10Reactome: R-HSA-1250342Reactome: R-HSA-1250347Reactome: R-HSA-1251985Reactome: R-HSA-1257604 Q02297-10Reactome: R-HSA-1306955 Q02297-10Reactome: R-HSA-1358803 Q02297-10Reactome: R-HSA-1963640Reactome: R-HSA-1963642 Q02297-10Reactome: R-HSA-2219530 Q02297-10Reactome: R-HSA-5673001 Q02297-10Reactome: R-HSA-6785631 Q02297-10Reactome: R-HSA-6811558 Q02297-10Reactome: R-HSA-8847993 Q02297-10Reactome: R-HSA-8863795 Q02297-10Reactome: R-HSA-9620244Reactome: R-HSA-9664565 Q02297-10Reactome: R-HSA-9665686 Q02297-10 |
| Cellular Localisation | Pro-Neuregulin-1Membrane-Bound Isoform: Cell MembraneSingle-Pass Type I Membrane ProteinDoes Not Seem To Be ActiveNeuregulin-1: SecretedIsoform 8: NucleusMay Be NuclearIsoform 9: SecretedHas A Signal PeptideIsoform 10: MembraneMay Possess An Internal Uncleaved Signal Sequence |
| Alternative CLIA Names | Pro-Neuregulin-1 - Membrane-Bound Isoform CLIA kitPro-Nrg1 Cleaved Into - Neuregulin-1 CLIA kitAcetylcholine Receptor-Inducing Activity CLIA kitAria CLIA kitBreast Cancer Cell Differentiation Factor P45 CLIA kitGlial Growth Factor CLIA kitHeregulin CLIA kitHrg CLIA kitNeu Differentiation Factor CLIA kitSensory And Motor Neuron-Derived Factor CLIA kitNRG1 CLIA kitGGF CLIA kitHGL CLIA kitHRGA CLIA kitNDF CLIA kitSMDF CLIA kit |
| Specificity | This kit recognizes Human NRG-1 in samples. No significant cross-reactivity or interference between Human NRG-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 96-112 | 103 |
| EDTA plasma(n=8) | 97-114 | 104 |
| Cell culture media(n=8) | 89-102 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 94.05 | 218.77 | 834.79 | 87.98 | 199.80 | 768.90 |
| Standard deviation | 10.40 | 16.63 | 52.09 | 8.09 | 17.70 | 78.20 |
| CV (%) | 11.06 | 7.60 | 6.24 | 9.20 | 8.86 | 10.17 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
