• Human H4 (Histone H4) Sandwich ELISA Kit (STJE0009378)

Human H4 (Histone H4) Sandwich ELISA Kit (STJE0009378)

SKU:
STJE0009378

Current Stock:
Applications: ELISA
Reactivity: Human
Note: FOR RESEARCH USE ONLY (RUO).
Sensitivity: 0.053ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This H4 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: H4C1.H4C2.H4C3.H4C4.H4C5.H4C6.H4C8.H4C9.H4C11.H4C12.H4C13.H4C14.H4C15.H4-16
Gene ID: 121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8370
Uniprot ID: H4_HUMAN
Sample Type: serum, plasma, tissue homogenates or other biological fluids.
Tissue Specificity
Post Translational Modifications Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by KMT5A/SET8. Dimethylation and trimethylation is performed by KMT5B and KMT5C and induces gene silencing. Monomethylated at Lys-13 (H4K12me1) by N6AMT1.H4K12me1 modification is present at the promoters of numerous genes encoding cell cycle regulators. Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ufmylated.monofmylated by UFL1 at Lys-32 (H4K31Ufm1) in response to DNA damage. Sumoylated, which is associated with transcriptional repression. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Butyrylation of histones marks active promoters and competes with histone acetylation. Glutarylation at Lys-92 (H4K91glu) destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription.
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein Name Histone H4
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-1221632
Reactome: R-HSA-171306
Reactome: R-HSA-1912408
Reactome: R-HSA-201722
Reactome: R-HSA-212300
Reactome: R-HSA-2299718
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-3214815
Reactome: R-HSA-3214841
Reactome: R-HSA-3214842
Reactome: R-HSA-3214847
Reactome: R-HSA-3214858
Reactome: R-HSA-427359
Reactome: R-HSA-427389
Reactome: R-HSA-427413
Reactome: R-HSA-4551638
Reactome: R-HSA-5250924
Reactome: R-HSA-5334118
Reactome: R-HSA-5578749
Reactome: R-HSA-5617472
Reactome: R-HSA-5625886
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-606279
Reactome: R-HSA-69473
Reactome: R-HSA-73728
Reactome: R-HSA-73772
Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-912446
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9616222
Reactome: R-HSA-9670095
Reactome: R-HSA-9710421
Reactome: R-HSA-977225
Cellular Localisation Nucleus
Chromosome
Alternative ELISA Names Histone H4 ELISA kit
H4C1 ELISA kit
H4 ELISA kit
A ELISA kit
H4FA ELISA kit
HIST1H4A.H4C2 ELISA kit
H4 ELISA kit
I ELISA kit
H4FI ELISA kit
HIST1H4B.H4C3 ELISA kit
H4 ELISA kit
G ELISA kit
H4FG ELISA kit
HIST1H4C.H4C4 ELISA kit
H4 ELISA kit
B ELISA kit
H4FB ELISA kit
HIST1H4D.H4C5 ELISA kit
H4 ELISA kit
J ELISA kit
H4FJ ELISA kit
HIST1H4E.H4C6 ELISA kit
H4 ELISA kit
C ELISA kit
H4FC ELISA kit
HIST1H4F.H4C8 ELISA kit
H4 ELISA kit
H ELISA kit
H4FH ELISA kit
HIST1H4H.H4C9 ELISA kit
H4 ELISA kit
M ELISA kit
H4FM ELISA kit
HIST1H4I.H4C11 ELISA kit
H4 ELISA kit
E ELISA kit
H4FE ELISA kit
HIST1H4J.H4C12 ELISA kit
H4 ELISA kit
D ELISA kit
H4FD ELISA kit
HIST1H4K.H4C13 ELISA kit
H4 ELISA kit
K ELISA kit
H4FK ELISA kit
HIST1H4L.H4C14 ELISA kit
H4 ELISA kit
N ELISA kit
H4F2 ELISA kit
H4FN ELISA kit
HIST2H4 ELISA kit
HIST2H4A.H4C15 ELISA kit
H4 ELISA kit
O ELISA kit
H4FO ELISA kit
HIST2H4B.H4-16 ELISA kit
HIST4H4 ELISA kit
output

Information sourced from Uniprot.org

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