| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This GHR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
GHR |
| Gene ID: |
2690 |
| Uniprot ID: |
GHR_HUMAN |
| Specificity: |
This kit recognizes Human GHR in samples. No significant cross-reactivity or interference between Human GHR and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17. On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome. Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of growth hormone receptor signaling. Ubiquitination is not sufficient for GHR internalization. |
| Function | Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Growth hormone-binding protein: The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2: Up-regulates the production of the soluble Growth hormone-binding protein form (GHBP) and acts as a negative inhibitor of growth hormone signaling. |
| Protein Name | Growth Hormone ReceptorGh ReceptorSomatotropin Receptor Cleaved Into - Growth Hormone-Binding ProteinGh-Binding ProteinGhbpSerum-Binding Protein |
| Database Links | Reactome: R-HSA-1170546Reactome: R-HSA-982772 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinOn Growth Hormone BindingGhr Is UbiquitinatedInternalizedDown-Regulated And Transported Into A Degradative Or Non-Degradative PathwayIsoform 2: Cell MembraneRemains Fixed To The Cell Membrane And Is Not InternalizedGrowth Hormone-Binding Protein: SecretedComplexed To A Substantial Fraction Of Circulating Gh |
| Alternative ELISA Names | Growth Hormone Receptor ELISA kitGh Receptor ELISA kitSomatotropin Receptor Cleaved Into - Growth Hormone-Binding Protein ELISA kitGh-Binding Protein ELISA kitGhbp ELISA kitSerum-Binding Protein ELISA kitGHR ELISA kit |
| Specificity | This kit recognizes Human GHR in samples. No significant cross-reactivity or interference between Human GHR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-96 | 90 |
| EDTA plasma (n=8) | 95-109 | 102 |
| Cell culture media (n=8) | 93-111 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 5.40 | 12.54 | 38.08 | 5.76 | 13.64 | 38.32 |
| Standard deviation | 0.37 | 0.56 | 1.94 | 0.31 | 0.71 | 1.36 |
| CV (%) | 6.85 | 4.47 | 5.09 | 5.38 | 5.21 | 3.55 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
