Flow Cytometry Gating
Loading Control Antibody Guide
Knockout-validated primary antibodies
Secondary antibodies resources
Alexa Fluor secondary antibodies
Biotinylated secondary antibodies
Gold-labelled secondary antibodies
How to prepare Phosphate Buffered Saline (PBS)
How to design a flow cytometry panel
Enhancing Detection of Low-Abundance Proteins
9 tips for detecting phosphorylation events using a Western Blot
Western Blotting with Tissue Lysates
Western blot membrane stripping protocol
Immunohistochemistry and Immunocytochemistry
Chromogenic and Fluorescent detection
Preparing paraffin-embedded and frozen samples for Immunohistochemistry
Competitive ELISA assay protocol
Measuring analyte concentration using serial dilutions and standard curve
What is Flow Cytometry gating?
Gating is an important part of any flow cytometry experiment. The gates alongside regions can be placed around cells expressing similar characteristics, often forward and side scatter or fluorescent marker expression, in order to examine and quantify these target populations.
It is best to obtain as much information about the cells you will be observing and the controls and markers used prior to the start of your experiment. One way to do so is to estimate the size and granularity of the cells and how these parameters can change over the course of the experiment due to apoptosis, intracellular staining or fixation.
By adding a negative control, you can easily set up a negative gate and observe only the real populations, which further simplifies the experiment.