• Immunohistochemistry of paraffin-embedded rat brain using PTEN antibody (STJ29874) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse brain using PTEN antibody (STJ29874) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded human stomach using PTEN antibody (STJ29874) at dilution of 1:200 (40x lens).
  • Immunofluorescence analysis of C6 cells using PTEN antibody (STJ29874) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PTEN antibody (STJ29874) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using PTEN antibody (STJ29874) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PTEN-antibody (aa190-304) (STJ29874)

SKU:
STJ29874

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Host: Rabbit
Applications: IHC, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-PTEN is suitable for use in Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: PTEN
Gene ID: 5728
Uniprot ID: PTEN_HUMAN
Immunogen Region: 190-304
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-304 of human PTEN (NP_000305.3).
Tissue Specificity Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Post Translational Modifications Constitutively phosphorylated by CK2 under normal conditions. Phosphorylated in vitro by MAST1, MAST2, MAST3 and STK11. Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both inhibit or promote PDZ-binding. Phosphorylation at Tyr-336 by FRK/PTK5 protects this protein from ubiquitin-mediated degradation probably by inhibiting its binding to NEDD4. Phosphorylation by ROCK1 is essential for its stability and activity. Phosphorylation by PLK3 promotes its stability and prevents its degradation by the proteasome. Monoubiquitinated.monoubiquitination is increased in presence of retinoic acid. Deubiquitinated by USP7.leading to its nuclear exclusion. Monoubiquitination of one of either Lys-13 and Lys-289 amino acid is sufficient to modulate PTEN compartmentalization. Ubiquitinated by XIAP/BIRC4.
Function Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement. Isoform alpha: Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1.
Protein Name Phosphatidylinositol 3 -4 -5-Trisphosphate 3-Phosphatase And Dual-Specificity Protein Phosphatase Pten
Mutated In Multiple Advanced Cancers 1
Phosphatase And Tensin Homolog
Database Links Reactome: R-HSA-1660499
Reactome: R-HSA-1855204
Reactome: R-HSA-199418
Reactome: R-HSA-202424
Reactome: R-HSA-5628897
Reactome: R-HSA-5674404
Reactome: R-HSA-5689880
Reactome: R-HSA-5689896
Reactome: R-HSA-8943723
Reactome: R-HSA-8948747
Reactome: R-HSA-8948751
Reactome: R-HSA-8986944
Cellular Localisation Cytoplasm
Nucleus
Pml Body
Monoubiquitinated Form Is Nuclear
Nonubiquitinated Form Is Cytoplasmic
Colocalized With Pml And Usp7 In Pml Nuclear Bodies
Xiap/Birc4 Promotes Its Nuclear Localization
Isoform Alpha: Secreted
May Be Secreted Via A Classical Signal Peptide And Reenter Into Cells With The Help Of A Poly-Arg Motif
Alternative Antibody Names Anti-Phosphatidylinositol 3 -4 -5-Trisphosphate 3-Phosphatase And Dual-Specificity Protein Phosphatase Pten antibody
Anti-Mutated In Multiple Advanced Cancers 1 antibody
Anti-Phosphatase And Tensin Homolog antibody
Anti-PTEN antibody
Anti-MMAC1 antibody
Anti-TEP1 antibody

Information sourced from Uniprot.org

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