• Immunofluorescence analysis of HeLa cells using MAP-2 monoclonal antibody.
  • Western blot analysis using MAP-2 monoclonal antibody against mouse brain, HeLa cell lysate.

Anti-MAP-2 antibody (STJ98517)

SKU:
STJ98517
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Host: Mouse
Applications: WB, IF
Reactivity: Human,Mouse,Rat,Bovine,Pig
Short Description: Mouse monoclonal primary antibody against MAP-2.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Conjugation: Unconjugated
Formulation: Buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Purification: Affinity purification
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Dilution Range: WB 1:1000-1:2000
IF 1:100-1:500
Specificity: MAP-2 monoclonal antibody detects endogenous levels of MAP-2 protein.
Uniprot ID: MTAP2_HUMAN
Gene ID: 4133
Gene Symbol: MAP2
Immunogen: Purified recombinant human MAP-2 (N-terminal) protein fragments expressed in E.coli.
Immunogen Region: N-terminal
Post Translational Modifications Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation.
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Dendrite
Protein Name Microtubule-Associated Protein 2
Map-2
Alternative Names Anti-Microtubule-Associated Protein 2 antibody
Anti-Map-2 antibody
Anti-MAP2 antibody

Information sourced from uniprot.org

6 months standard