• Immunohistochemistry analysis of paraffin-embedded human brain, using CLUS Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from HeLa cells and A549 cells, using CLUS Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunofluorescence analysis of HeLa cells, using CLUS Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of various cells using Clusterin Polyclonal Antibody
  • Immunofluorescence analysis of Hela cell. 1, Clusterin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CLU antibody (370-450 C-Term) (STJ92346)

SKU:
STJ92346

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ICC, ELISA
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-Clusterin (370-450 C-Term) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLU
Gene ID: 1191
Uniprot ID: CLUS_HUMAN
Immunogen Region: 370-450 C-Term
Specificity: CLU polyclonal antibody (Clusterin) binds to endogenous Clusterin at the amino acid region 370-450 C-Term.
Immunogen: The antiserum was produced against synthesized peptide derived from human CLU at amino acid range 400-449
Post Translational Modifications Proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen. Proteolytic cleavage is not necessary for its chaperone activity. All non-secreted forms are not proteolytically cleaved. Chaperone activity of uncleaved forms is dependent on a non-reducing envoronment. Polyubiquitinated, leading to proteasomal degradation. Under cellular stress, the intracellular level of cleaved form is reduced due to proteasomal degradation. Extensively glycosylated with sulfated N-linked carbohydrates. About 30% of the protein mass is comprised of complex N-linked carbohydrate. Endoplasmic reticulum (ER) stress induces changes in glycosylation status and increases level of hypoglycosylated forms. Core carbohydrates are essential for chaperone activity. Non-secreted forms are hypoglycosylated or unglycosylated.
Function Isoform 1: Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. A mitochondrial form suppresses BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3. Plays a role in the clearance of immune complexes that arise during cell injury. Isoform 6: Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Isoform 4: Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Promotes cell death through interaction with BCL2L1 that releases and activates BAX.
Protein Name Clusterin
Aging-Associated Gene 4 Protein
Apolipoprotein J
Apo-J
Complement Cytolysis Inhibitor
Cli
Complement-Associated Protein Sp-40 -40
Ku70-Binding Protein 1
Na1/Na2
Sulfated Glycoprotein 2
Sgp-2
Testosterone-Repressed Prostate Message 2
Trpm-2 Cleaved Into - Clusterin Beta Chain
Apojalpha
Complement Cytolysis Inhibitor A Chain - Clusterin Alpha Chain
Apojbeta
Complement Cytolysis Inhibitor B Chain
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-166665
Reactome: R-HSA-6803157
Reactome: R-HSA-977606
Cellular Localisation Isoform 1: Secreted
Can Retrotranslocate From The Secretory Compartments To The Cytosol Upon Cellular Stress
Isoform 4: Cytoplasm
Keeps Cytoplasmic Localization In Stressed And Unstressed Cell
Isoform 6: Cytoplasm
Nucleus
Cytoplasm
Mitochondrion Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytosol
Microsome
Endoplasmic Reticulum
Mitochondrion
Perinuclear Region
Cytoplasmic Vesicle
Secretory Vesicle
Chromaffin Granule
Secreted Isoforms Can Retrotranslocate From The Secretory Compartments To The Cytosol Upon Cellular Stress
Detected In Perinuclear Foci That May Be Aggresomes Containing Misfolded
Ubiquitinated Proteins
Detected At The Mitochondrion Membrane Upon Induction Of Apoptosis
Under Er Stress
A Immaturely Glycosylated Pre-Secreted Form Retrotranslocates From The Endoplasmic Reticulum (Er)-Golgi Network To The Cytoplasm To Localize In The Mitochondria Through Hspa5 Interaction
Er Stress Reduces Secretion
Under The Stress
Minor Amounts Of Non-Secreted Forms Accumulate In Cytoplasm
Non-Secreted Forms Emerge Mainly From Failed Translocation
Alternative Splicing Or Non-Canonical Initiation Start Codon
Alternative Antibody Names Anti-Clusterin antibody
Anti-Aging-Associated Gene 4 Protein antibody
Anti-Apolipoprotein J antibody
Anti-Apo-J antibody
Anti-Complement Cytolysis Inhibitor antibody
Anti-Cli antibody
Anti-Complement-Associated Protein Sp-40 -40 antibody
Anti-Ku70-Binding Protein 1 antibody
Anti-Na1/Na2 antibody
Anti-Sulfated Glycoprotein 2 antibody
Anti-Sgp-2 antibody
Anti-Testosterone-Repressed Prostate Message 2 antibody
Anti-Trpm-2 Cleaved Into - Clusterin Beta Chain antibody
Anti-Apojalpha antibody
Anti-Complement Cytolysis Inhibitor A Chain - Clusterin Alpha Chain antibody
Anti-Apojbeta antibody
Anti-Complement Cytolysis Inhibitor B Chain antibody
Anti-CLU antibody
Anti-APOJ antibody
Anti-CLI antibody
Anti-KUB1 antibody
Anti-AAG4 antibody

Information sourced from Uniprot.org

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