• Immunohistochemistry of paraffin-embedded human liver damage using CHMP1B antibody (STJ110538) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse kidney using CHMP1B antibody (STJ110538) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat brain using CHMP1B antibody (STJ110538) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using CHMP1B antibody (STJ110538) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-CHMP1B antibody (STJ110538)

SKU:
STJ110538

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Host: Rabbit
Applications: WB, IHC
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-CHMP1B antibody is suitable for use in Western Blot and Immunohistochemistry.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:100
Uniprot ID: CHM1B_HUMAN
Gene ID: 57132
Gene Symbol: CHMP1B
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human CHMP1B (NP_065145.2).
Tissue Specificity Widely expressed. Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta and brain.
Function Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B and SPAST to the midbody of dividing cells. Involved in HIV-1 p6- and p9-dependent virus release.
Protein Name Charged Multivesicular Body Protein 1b
Chmp1.5
Chromatin-Modifying Protein 1b
Chmp1b
Vacuolar Protein Sorting-Associated Protein 46-2
Vps46-2
Hvps46-2
Cellular Localisation Cytoplasm
Cytosol
Endosome
Late Endosome Membrane
Peripheral Membrane Protein
Localizes To The Midbody Of Dividing Cells
Colocalizing With Cep55 And Chmp5
Localized At The Periphery Of The Fleming Body
Alternative Antibody Names Anti-Charged Multivesicular Body Protein 1b antibody
Anti-Chmp1.5 antibody
Anti-Chromatin-Modifying Protein 1b antibody
Anti-Chmp1b antibody
Anti-Vacuolar Protein Sorting-Associated Protein 46-2 antibody
Anti-Vps46-2 antibody
Anti-Hvps46-2 antibody
Anti-CHMP1B antibody
Anti-C18orf2 antibody

Information sourced from Uniprot.org

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