![Anti-CD274/PD-L1/B7-H1 nanobody [SAA0904] (STJN000309)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/200922/413104/STJN000309-SDS-PAGE-1__32995.1749232604.jpg?c=1 "Anti-CD274/PD-L1/B7-H1 nanobody [SAA0904] (STJN000309)")
![Anti-CD274/PD-L1/B7-H1 nanobody [SAA0904] (STJN000309)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/200922/413104/STJN000309-SDS-PAGE-1__32995.1749232604.jpg?c=1 "Anti-CD274/PD-L1/B7-H1 nanobody [SAA0904] (STJN000309)")
Anti-CD274/PD-L1/B7-H1 nanobody [SAA0904] (STJN000309)
SPECIFICATIONS
ClonalityMonoclonal
HostAlpaca
ConjugationUnconjugated
IsotypeVHH-8His-Cys-tag
General Information
| Short Description | Alpaca monoclonal nanobody anti-Programmed Cell Death 1 Ligand 1 is suitable for use in ELISA research applications. |
| Applications | ELISA |
| Host | Alpaca |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA0904 |
| Isotype | VHH-8His-Cys-tag |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by Nickel column. |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at-20°C 12 months. Store at-80°C long term. |
Target Information
| Gene Symbol | CD274 |
| Gene ID | 29126 |
| Uniprot ID | PD1L1_HUMAN |
Additional Info
| Post Translational Modifications | Ubiquitinated.STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation. Ubiquitinated by MARCHF8.leading to degradation. Deubiquitinated by USP22.leading to stabilization. |
| Function | Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). Can also act as a transcription coactivator: in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. |
| Protein Name | Programmed Cell Death 1 Ligand 1Pd-L1Pdcd1 Ligand 1Programmed Death Ligand 1Hpd-L1B7 Homolog 1B7-H1Cd Antigen Cd274 |
| Database Links | Reactome: R-HSA-389948Reactome: R-HSA-9701898 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEarly Endosome MembraneRecycling Endosome MembraneNucleusAssociates With Cmtm6 At Recycling EndosomesWhere It Is Protected From Being Targeted For Lysosomal DegradationTranslocates To The Nucleus In Response To Hypoxia Via Its Interaction With Phosphorylated Stat3Isoform 1: Cell MembraneIsoform 2: Endomembrane SystemIsoform 4: Secreted |
| Alternative Nanobody Names | Anti-Programmed Cell Death 1 Ligand 1 nanobodyAnti-Pd-L1 nanobodyAnti-Pdcd1 Ligand 1 nanobodyAnti-Programmed Death Ligand 1 nanobodyAnti-Hpd-L1 nanobodyAnti-B7 Homolog 1 nanobodyAnti-B7-H1 nanobodyAnti-Cd Antigen Cd274 nanobodyAnti-CD274 nanobodyAnti-B7H1 nanobodyAnti-PDCD1L1 nanobodyAnti-PDCD1LG1 nanobodyAnti-PDL1 nanobody |
Information sourced from Uniprot.org