• Western blot analysis of A549 cells using Acetyl-ATF-5 (K29) Polyclonal Antibody. Secondary antibody was diluted at 1:20000
  • Western blot analysis of lysate from A549 cells, using ATF5 (Acetyl-Lys29) Antibody.
  • Western blot analysis of A549 cells using Acetyl-ATF-5 (K29) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-ATF5-Lys29 antibody (1-50) (STJ90139)


Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal antibody anti-Acetyl-Cyclic Amp-Dependent Transcription Factor Atf-5-Lys29 (1-50) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATF5
Gene ID: 22809
Uniprot ID: ATF5_HUMAN
Immunogen Region: 1-50
Specificity: Acetyl-ATF5-Lys29 polyclonal antibody (Cyclic Amp-Dependent Transcription Factor Atf-5) binds to endogenous Cyclic Amp-Dependent Transcription Factor Atf-5 at the amino acid region 1-50.
Immunogen: The antiserum was produced against synthesized Acetyl-peptide derived from human ATF5 around the Acetylation site of Lys29 at amino acid range 1-50
Post Translational Modifications Ubiquitinated by CDC34 and UBE2B in order to be degraded by the proteasome. Cisplatin inhibits ubiquitination and proteasome-mediated degradation by inhibiting the interaction with CDC34. Ubiquitination and degradation by the proteasome are inhibited by NLK in a kinase-independent manner. Phosphorylated by NLK, probably at Ser-92, Thr-94, Ser-126 and Ser-190. Acetylated at Lys-29 by EP300, the acetylation enhances the interaction with CEBPB, DNA-binding and transactivation activity.
Function Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGTACAG-3'), ATF5-specific response element (ARE) (consensus: 5'-CCTTCTCTCCTTAT-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate. Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation. Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes. May be involved in osteogenic differentiation. Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1. Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent. Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver. In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase. May act as a negative regulator of IL1B transduction pathway in liver. Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members. Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation. Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material.
Protein Name Cyclic Amp-Dependent Transcription Factor Atf-5
Camp-Dependent Transcription Factor Atf-5
Activating Transcription Factor 5
Transcription Factor Atfx
Database Links Reactome: R-HSA-9648895
Cellular Localisation Cytoplasm
Microtubule Organizing Center
Actively Transported To The Centrosome And Accumulated In The Pericentriolar Material (Pcm) During G1 To M Phase Via A Microtubule-Dependent Mechanism
During Late Telophase And Cytokinesis
Translocates From The Centrosome To The Midbody
Alternative Antibody Names Anti-Cyclic Amp-Dependent Transcription Factor Atf-5 antibody
Anti-Camp-Dependent Transcription Factor Atf-5 antibody
Anti-Activating Transcription Factor 5 antibody
Anti-Transcription Factor Atfx antibody
Anti-ATF5 antibody
Anti-ATFX antibody

Information sourced from Uniprot.org

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