• Western blot analysis of extracts of A-431 cells, using 53BP1 antibody (STJ28324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 40s.
  • Western blot analysis of extracts from normal (control) and 53BP1 knockout (KO) 293T cells, using 53BP1 antibody (STJ28324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded human esophageal using 53BP1 Rabbit pAb (STJ28324) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human brain using 53BP1 Rabbit pAb (STJ28324) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human brain using 53BP1 Rabbit pAb (STJ28324) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human esophageal using 53BP1 Rabbit pAb (STJ28324) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human brain using 53BP1 Rabbit pAb (STJ28324) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human brain using 53BP1 Rabbit pAb (STJ28324) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of U2OS cells using 53BP1 antibody (STJ28324). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using 53BP1 antibody (STJ28324). Green:GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Anti-53BP1-antibody (aa1763-1977) (STJ28324)

SKU:
STJ28324

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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-53BP1 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: TP53BP1
Gene ID: 7158
Uniprot ID: TP53B_HUMAN
Immunogen Region: 1763-1977
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1763-1977 of human 53BP1 (NP_001135452.1).
Function Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Participates in the repair and the orientation of the broken DNA ends during CSR. In contrast, it is not required for classic NHEJ and V(D)J recombination. Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1.
Protein Name Tp53-Binding Protein 1
53bp1
P53-Binding Protein 1
P53bp1
Database Links Reactome: R-HSA-3232118
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-69473
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Localizes To The Nucleus In Absence Of Dna Damage
Following Dna Damage
Recruited To Sites Of Dna Damage
Such As Double Stand Breaks (Dsbs): Recognizes And Binds Histone H2a Monoubiquitinated At 'Lys-15' (H2ak15ub) And Histone H4 Dimethylated At 'Lys-20' (H4k20me2)
Two Histone Marks That Are Present At Dsbs Sites
Associated With Kinetochores During Mitosis
Alternative Antibody Names Anti-Tp53-Binding Protein 1 antibody
Anti-53bp1 antibody
Anti-P53-Binding Protein 1 antibody
Anti-P53bp1 antibody
Anti-TP53BP1 antibody

Information sourced from Uniprot.org

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