| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase B-raf-Ser602 (576-625 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BRAF |
| Gene ID: | 673 |
| Uniprot ID: | BRAF_HUMAN |
| Immunogen Region: | 576-625 aa |
| Specificity: | Phospho-Raf-B (S602) Polyclonal Antibody detects endogenous levels of Raf-B protein only when phosphorylated at S602. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human B-RAF around the phosphorylation site of Ser602 at the amino acid range 576-625 |
| Post Translational Modifications | Phosphorylation at Ser-365 by SGK1 inhibits its activity (Ref.8, PubMed:11410590). Phosphorylation at Thr-753 by MAPK1. Dephosphorylation of Ser-365 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC).this relieves inactivation and stimulates kinase activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149.which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
| Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons. |
| Protein Name | Serine/Threonine-Protein Kinase B-Raf Proto-Oncogene B-Raf P94 V-Raf Murine Sarcoma Viral Oncogene Homolog B1 |
| Database Links | Reactome: R-HSA-1295596 Reactome: R-HSA-170968 Reactome: R-HSA-170984 Reactome: R-HSA-187706 Reactome: R-HSA-5673000 Reactome: R-HSA-5674135 Reactome: R-HSA-5674499 Reactome: R-HSA-5675221 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9726840 Reactome: R-HSA-9726842 |
| Cellular Localisation | Nucleus Cytoplasm Cell Membrane Colocalizes With Rgs14 And Raf1 In Both The Cytoplasm And Membranes |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase B-Raf antibody Anti-Proto-Oncogene B-Raf antibody Anti-P94 antibody Anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 antibody Anti-BRAF antibody Anti-BRAF1 antibody Anti-RAFB1 antibody |
Information sourced from Uniprot.org