||FOR RESEARCH USE ONLY (RUO).
||This Rat PRF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
||Serum, Plasma, Cell supernatant
| Background || This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PRF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PRF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PRF1, biotinylated detection antibody and Avidin-HRP conjugate will develop fluorescent colour. The Relative Light Unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PRF1. You can calculate the concentration of Rat PRF1 in the samples by comparing the RLU value of the samples to the standard curve. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance