• Rat PRF1 (Perforin 1) CLIA Kit (STJC0001833)

Rat PRF1 (Perforin 1) CLIA Kit (STJC0001833)

SKU:
STJC0001833

£238.50 - £477.50
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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This rat PRF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Prf1Uniprot ID=PERF_RAT"
Specificity: This kit recognizes Rat PRF1 in samples. No significant cross-reactivity or interference between Rat PRF1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Detected in large granular lymphocytes and lymphokine-activated killer cells.
Post Translational Modifications N-glycosylated.
Function Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu.
Protein Name Perforin-1
P1
Cytolysin
Lymphocyte Pore-Forming Protein
Cellular Localisation Cytolytic Granule
Secreted
Cell Membrane
Multi-Pass Membrane Protein
Endosome Lumen
Stored In Cytolytic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target Cell
Inserts Into The Cell Membrane Of Target Cells And Forms Pores
Membrane Insertion And Pore Formation Requires A Major Conformation Change
May Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes
Alternative CLIA Names Perforin-1 CLIA kit
P1 CLIA kit
Cytolysin CLIA kit
Lymphocyte Pore-Forming Protein CLIA kit
Prf1 CLIA kit
Pfp CLIA kit
Specificity This kit recognizes Rat PRF1 in samples. No significant cross-reactivity or interference between Rat PRF1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-104 95
EDTA plasma(n=8) 90-102 97
Cell culture media(n=8) 84-97 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 215.91 619.45 1357.54 209.82 567.98 1404.47
Standard deviation 22.80 58.79 95.30 25.60 64.64 109.55
CV (%) 10.56 9.49 7.02 12.20 11.38 7.80
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance