Rat Mbp (Myelin basic protein) Sandwich ELISA Kit (STJE0005916)

Name: Rat Mbp (Myelin basic protein) Sandwich ELISA Kit (STJE0005916)
SKU: STJE0005916
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0005916

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Short Description	This Mbp Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Rat
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Mbp
Gene ID	24547
Uniprot ID	MBP_RAT
Specificity	This kit recognizes Rat MBP in samples. No significant cross-reactivity or interference between Rat MBP and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	As in other animals, several charge isomers may be produced as a result of optional post-translational modifications, such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. Arg-131 was found to be 44% monomethylated and 11% symmetrically dimethylated. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1. Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells.
Function	Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane.
Protein Name	Myelin Basic Protein Mbp
Database Links
Cellular Localisation	Myelin Membrane Peripheral Membrane Protein Cytoplasmic Side Cytoplasmic Side Of Myelin
Alternative ELISA Names	Myelin Basic Protein ELISA kit Mbp ELISA kit Mbp ELISA kit
Specificity	This kit recognizes Rat MBP in samples. No significant cross-reactivity or interference between Rat MBP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.50	1.25	4.11	0.53	1.31	4.32
Standard deviation	0.03	0.07	0.14	0.03	0.07	0.20
CV (%)	6.00	5.60	3.41	5.66	5.34	4.63