• Rabbit SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit (STJE0005417)

Rabbit SOD1 (Superoxide Dismutase 1, Soluble) ELISA Kit (STJE0005417)

Current Stock:
Applications: ELISA
Reactivity: Rabbit
Short Description: This SOD1 Competitive ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Detection: Colorimetric
Sample Type: Serum, Plasma, Cell supernatant
Background This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rabbit SOD1. During the reaction, Rabbit SOD1 in the sample or standard competes with a fixed amount of Rabbit SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rabbit SOD1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rabbit SOD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Information sourced from Uniprot.org

6 months standard