Mouse Cxcl9 (C-X-C motif chemokine 9) Sandwich ELISA Kit (STJE0004217)

SKU:
STJE0004217

Current Stock:
Applications: ELISA
Reactivity: Mouse
Note: FOR RESEARCH USE ONLY (RUO).
Sensitivity: 28.13pg/mL
Detection Limit: 46.88~3000pg/mL
Short Description: This Cxcl9 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8 °C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Cxcl9
Gene ID: 17329
Uniprot ID: CXCL9_MOUSE
Specificity: This kit recognizes Mouse MI Gamma/CXCL9 in samples. No significant cross-reactivity or interference between Mouse MI Gamma/CXCL9 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function May be a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response.
Protein Name C-X-C Motif Chemokine 9
Gamma-Interferon-Induced Monokine
Monokine Induced By Interferon-Gamma
Mig
Mumig
Protein M119
Small-Inducible Cytokine B9
Database Links Reactome: R-MMU-380108
Reactome: R-MMU-418594
Cellular Localisation Secreted
Alternative ELISA Names C-X-C Motif Chemokine 9 ELISA kit
Gamma-Interferon-Induced Monokine ELISA kit
Monokine Induced By Interferon-Gamma ELISA kit
Mig ELISA kit
Mumig ELISA kit
Protein M119 ELISA kit
Small-Inducible Cytokine B9 ELISA kit
Cxcl9 ELISA kit
Mig ELISA kit
Scyb9 ELISA kit
Specificity This kit recognizes Mouse MIγ/CXCL9 in samples. No significant cross-reactivity or interference between Mouse MIγ/CXCL9 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 96
EDTA plasma (n=8) 90-105 97
Cell culture media (n=8) 92-110 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 156.80 445.80 1497.30 154.10 484.80 1422.10
Standard deviation 10.70 23.60 46.40 9.70 22.80 58.30
CV (%) 6.82 5.29 3.10 6.29 4.70 4.10
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance