| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR RESEARCH USE ONLY (RUO). |
| Sensitivity: |
28.13pg/mL |
| Detection Limit: |
46.88~3000pg/mL |
| Short Description: |
This Cxcl9 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8 °C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Cxcl9 |
| Gene ID: |
17329 |
| Uniprot ID: |
CXCL9_MOUSE |
| Specificity: |
This kit recognizes Mouse MI Gamma/CXCL9 in samples. No significant cross-reactivity or interference between Mouse MI Gamma/CXCL9 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | May be a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. |
| Protein Name | C-X-C Motif Chemokine 9Gamma-Interferon-Induced MonokineMonokine Induced By Interferon-GammaMigMumigProtein M119Small-Inducible Cytokine B9 |
| Database Links | Reactome: R-MMU-380108Reactome: R-MMU-418594 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | C-X-C Motif Chemokine 9 ELISA kitGamma-Interferon-Induced Monokine ELISA kitMonokine Induced By Interferon-Gamma ELISA kitMig ELISA kitMumig ELISA kitProtein M119 ELISA kitSmall-Inducible Cytokine B9 ELISA kitCxcl9 ELISA kitMig ELISA kitScyb9 ELISA kit |
| Specificity | This kit recognizes Mouse MIγ/CXCL9 in samples. No significant cross-reactivity or interference between Mouse MIγ/CXCL9 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-102 | 96 |
| EDTA plasma (n=8) | 90-105 | 97 |
| Cell culture media (n=8) | 92-110 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 156.80 | 445.80 | 1497.30 | 154.10 | 484.80 | 1422.10 |
| Standard deviation | 10.70 | 23.60 | 46.40 | 9.70 | 22.80 | 58.30 |
| CV (%) | 6.82 | 5.29 | 3.10 | 6.29 | 4.70 | 4.10 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
