Human SDF-1 alpha protein (Recombinant) {Biotin} (STJP000208)

SKU:
STJP000208

Current Stock:
Host: E.coli
Applications: ELISA, WB
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Recombinant-Human SDF-1 alpha-protein was developed from e.coli. For use in research applications.
Conjugation: Biotin
Formulation: Recombinant SDF-1 alpha was lyophilized from a.2 Mu m filtered PBS, pH.4.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: The lyophilized protein is stable for at least years from date of receipt at-20°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8°C for one month, or at-20°C for six months, with a carrier protein without detectable loss
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: CXCL12
Gene ID: 6387
Uniprot ID: SDF1_HUMAN
Immunogen Region: Mature chain
Immunogen Sequence: MKASAALLCL LLTAAAFSPQ GLAQPVGINT STTCCYRFIN KKIPKQRLES YRRTTSSHCP REAVIFKTKL DKEICADPTQ KWVQDFMKHL DKKTQTPKL
Tissue Specificity Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
Post Translational Modifications Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
Function Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development.required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells.
Protein Name Stromal Cell-Derived Factor 1
Sdf-1
Hsdf-1
C-X-C Motif Chemokine 12
Intercrine Reduced In Hepatomas
Irh
Hirh
Pre-B Cell Growth-Stimulating Factor
Pbsf Cleaved Into - Sdf-1-Beta(3-72 - Sdf-1-Alpha(3-67
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-376176
Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-9018519
Cellular Localisation Secreted
Alternative Antibody Names Anti-Stromal Cell-Derived Factor 1 antibody
Anti-Sdf-1 antibody
Anti-Hsdf-1 antibody
Anti-C-X-C Motif Chemokine 12 antibody
Anti-Intercrine Reduced In Hepatomas antibody
Anti-Irh antibody
Anti-Hirh antibody
Anti-Pre-B Cell Growth-Stimulating Factor antibody
Anti-Pbsf Cleaved Into - Sdf-1-Beta(3-72 - Sdf-1-Alpha(3-67 antibody
Anti-CXCL12 antibody
Anti-SDF1 antibody
Anti-SDF1A antibody
Anti-SDF1B antibody

Information sourced from Uniprot.org

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