| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human PDE4D kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
PDE4D |
| Gene ID: |
5144 |
| Uniprot ID: |
PDE4D_HUMAN |
| Specificity: |
This kit recognizes Human PDE4D in samples. No significant cross-reactivity or interference between Human PDE4D and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in colonic epithelial cells (at protein level). Widespread.most abundant in skeletal muscle. Isoform 6: Detected in brain. Isoform 8: Detected in brain, placenta, lung and kidney. Isoform 7: Detected in heart and skeletal muscle. |
| Post Translational Modifications | Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation. Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation. |
| Function | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
| Protein Name | Camp-Specific 3' -5'-Cyclic Phosphodiesterase 4dDpde3Pde43 |
| Database Links | Reactome: R-HSA-180024Reactome: R-HSA-418555 |
| Cellular Localisation | Apical Cell MembraneCytoplasmMembraneCytoskeletonMicrotubule Organizing CenterCentrosomeFound In The Soluble FractionAssociated With MembranesAnd Associated With The Cytoskeleton And The CentrosomeColocalized With Shank2 To The Apical Membrane Of Colonic Crypt Cells |
| Alternative CLIA Names | Camp-Specific 3' -5'-Cyclic Phosphodiesterase 4d CLIA kitDpde3 CLIA kitPde43 CLIA kitPDE4D CLIA kitDPDE3 CLIA kit |
| Specificity | This kit recognizes Human PDE4D in samples. No significant cross-reactivity or interference between Human PDE4D and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-101 | 93 |
| EDTA plasma(n=8) | 97-110 | 103 |
| Cell culture media(n=8) | 98-115 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 198.98 | 541.46 | 1580.65 | 182.00 | 569.31 | 1717.96 |
| Standard deviation | 16.06 | 44.13 | 139.73 | 20.49 | 56.36 | 165.61 |
| CV (%) | 8.07 | 8.15 | 8.84 | 11.26 | 9.90 | 9.64 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
