||FOR RESEARCH USE ONLY (RUO).
||Recombinant-Human HCC-1 (CCL14)-protein was developed from e.coli. For use in research applications.
||Lyophilised from a.2 Mu m filteredmM PB, 100mM NaCl solution pH.5. Upon receipt A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
||Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
||The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. Upon reconstitution, this cytokine can be stored in working aliquots at°C-°C for one month, or at-20°C for six months, with a carrier protein without detectable loss
||Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg).
||MKISVAAIPF FLLITIALGT KTESSSRGPY HPSECCFTYT TYKIPRQRIM DYYETNSQCS KPGIVFITKR GHSVCTNPSD KWVQDYIKDM KEN
| Tissue Specificity || Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma. |
| Post Translational Modifications || The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated.the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids. |
| Function || Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. |
| Protein Name || C-C Motif Chemokine 14Chemokine Cc-1/Cc-3Hcc-1/Hcc-3Hcc-1(1-74Ncc-2Small-Inducible Cytokine A14 Cleaved Into - Hcc-1(3-74 - Hcc-1(4-74 - Hcc-1(9-74 |
| Cellular Localisation || Secreted |
| Alternative Protein Names || C-C Motif Chemokine 14 proteinChemokine Cc-1/Cc-3 proteinHcc-1/Hcc-3 proteinHcc-1(1-74 proteinNcc-2 proteinSmall-Inducible Cytokine A14 Cleaved Into - Hcc-1(3-74 - Hcc-1(4-74 - Hcc-1(9-74 proteinCCL14 proteinNCC2 proteinSCYA14 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance