Human GRB10 protein (Recombinant) (N-His) (STJP005316)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human GRB10-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | GRB10 |
| Gene ID | 2887 |
| Uniprot ID | GRB10_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Thr392-Leu594 |
Additional Info
| Post Translational Modifications | Phosphorylated on serine residues upon EGF, FGF and PDGF stimulation. Phosphorylated at Tyr-67 by TEC. |
| Function | Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2. |
| Protein Name | Growth Factor Receptor-Bound Protein 10Grb10 Adapter ProteinInsulin Receptor-Binding Protein Grb-Ir |
| Database Links | Reactome: R-HSA-1433557Reactome: R-HSA-74713Reactome: R-HSA-74749Reactome: R-HSA-74751Reactome: R-HSA-8853659Reactome: R-HSA-9607240Reactome: R-HSA-9648895 |
| Cellular Localisation | CytoplasmWhen Complexed With Nedd4 And Igf1rFollows Igf1r InternalizationRemaining Associated With Early EndosomesUncouples From Igf1r-Containing Endosomes Before The Sorting Of The Receptor To The Lysosomal Compartment |
| Alternative Protein Names | Growth Factor Receptor-Bound Protein 10 proteinGrb10 Adapter Protein proteinInsulin Receptor-Binding Protein Grb-Ir proteinGRB10 proteinGRBIR proteinKIAA0207 protein |
Information sourced from Uniprot.org