| Applications: |
ELISA |
| Reactivity: |
Bovine/Sheep |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.96ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This Cortisol Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in bovine/sheep cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
2.5h |
| Detection: |
Colormetric |
| Specificity: |
This kit recognizes Bovine/Sheep Cortisol in samples. No significant cross-reactivity or interference between Bovine/Sheep Cortisol and analogues was observed. |
| Sample Type: |
Serum and plasma |
| Specificity | This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 81-90 | 85 |
| EDTA plasma(n=8) | 82-98 | 88 |
| Urine(n=8) | | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 40.54 | 100.33 | 208.22 | 41.23 | 102.25 | 205.71 |
| Standard deviation | 3.63 | 7.68 | 14.64 | 3.57 | 8.17 | 12.45 |
| CV (%) | 8.96 | 7.65 | 7.03 | 8.66 | 7.99 | 6.05 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
